SOLUTIONS

TOYOPEARL PPG-600M

TOYOPEARL PPG-600M

TOYOPEARL PPG-600M is based on a 75 nm (750 Å) pore size polymethacrylate base material bonded with polypropylene glycol groups. It has the second lowest hydrophobicity of the HIC ligands offered by Tosoh Bioscience. Its pore size has been optimized for the purification of monoclonal antibodies. It is typically used for intermediate purification or polishing steps. It has extremely good capacity and recovery for mAbs.

 

Structure of TOYOPEARL PPG-600M resins 

PPG_structure.png
 

 

Product attributes

Pore size (mean): 75 nm
Particle size (mean): 65 µm (M-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Comparison of TOYOPEARL -600M resins

HIC_600resins_fig1.png
 
Resins:            A. TOYOPEARL PPG-600M
                         B. TOYOPEARL Phenyl-600M
                         C. TOYOPEARL Butyl-600M
Column size:  7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO4 + 0.1 mol/L sodium phosphate, pH 7.0
                         Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient:        60 min linear gradient from buffer A to B
Flow rate:       136 cm/hr (1.0 mL/min)
Detection:       UV @ 280 nm
Temperature: ambient
Samples:        1 g/L of: 1. ribonuclease A, 2.lysozyme, 3. α-chymotrypsinogen
Load volume: 100 µL

Breakthrough curves of polyclonal IgG on various HIC resins

HIC_600resins_fig2.png

Resins:           A. TOYOPEARL PPG-600M
                        B. TOYOPEARL Phenyl-650M
                        C. TOYOPEARL Butyl-600M
                        D. Competitor Phenyl Agarose
                        E. TOYOPEARL Phenyl-600M
Column size:  7.8 mm ID × 20 cm
Mobile phase: 0.1 mol/L sodium phosphate, pH 7.0 + 0.8 mol/L (NH4)2SO4
Flow rate:       300 cm/hr (2.4 mL/min)
Detection:       UV @ 280 nm
Temperature: 25 °C
Samples:         1.0 g/L polyclonal IgG

DBC was calculated at 10% breakthrough

 

Ordering Information

P/N Description Dimension
0021301 TOYOPEARL PPG-600M 25 mL
0021302 TOYOPEARL PPG-600M 100 mL
0021303 TOYOPEARL PPG-600M 1 L
0021304 TOYOPEARL PPG-600M 5 L
0021380 TOYOPEARL PPG-600M 6 x 1 mL
0021381 TOYOPEARL PPG-600M 6 x 5 mL
0021398 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 1 mL
0021399 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 5 mL
0045124 MiniChrom TOYOPEARL PPG-600M, 5 mL 8 mm ID x 100 mm L
0045035 ToyoScreen RoboColumn PPG-600M 8 x 200 µL
0045036 ToyoScreen RoboColumn PPG-600M 8 x 600 µL