SOLUTIONS

TOYOPEARL Phenyl-600M

TOYOPEARL Phenyl-600M is based on a 75 nm (750 Å) pore size polymethacrylate base material bonded with phenyl groups. Its pore size has been optimized for the purification of monoclonal antibodies. It has an intermediate hydrophobicity, which is slightly higher than the one of the 100 nm pore size TOYOPEARL Phenyl-650.

TOYOPEARL Phenyl-600M is typically used for intermediate purification or polishing steps. It is a very high recovery and high dynamic capacity resin for both large and small proteins. When used as the polishing step up to an extra 5% yield may be seen compared to other Phenyl resins.

To see other products optimized for HIC purification of mAbs please see TOYOPEARL PPG-600 and TOYOEPARL Butyl-600 media.

 

Structure of TOYOPEARL Phenyl-600 resins 

Phenyl600_structure.png
 

 

Product attributes

Pore size (mean): 75 nm
Particle size (mean): 65 µm (M-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Comparison of TOYOPEARL-600M resins

HIC_600resins_fig1.png
 
Resins:            A. TOYOPEARL PPG-600M
                         B. TOYOPEARL Phenyl-600M
                         C. TOYOPEARL Butyl-600M
Column size:  7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO4 + 0.1 mol/L sodium phosphate, pH 7.0
                         Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient:        60 min linear gradient from buffer A to B
Flow rate:       136 cm/hr (1.0 mL/min)
Detection:       UV @ 280 nm
Temperature: ambient
Samples:        1 g/L of: 1. ribonuclease A, 2.lysozyme, 3. α-chymotrypsinogen
Load volume: 100 µL

Breakthrough curves of polyclonal IgG on various HIC resins

HIC_600resins_fig2.png

Resins:           A. TOYOPEARL PPG-600M
                        B. TOYOPEARL Phenyl-650M
                        C. TOYOPEARL Butyl-600M
                        D. Competitor Phenyl Agarose
                        E. TOYOPEARL Phenyl-600M
Column size:  7.8 mm ID × 20 cm
Mobile phase: 0.1 mol/L sodium phosphate, pH 7.0 + 0.8 mol/L (NH4)2SO4
Flow rate:       300 cm/hr (2.4 mL/min)
Detection:       UV @ 280 nm
Temperature: 25 °C
Samples:         1.0 g/L polyclonal IgG

DBC was calculated at 10% breakthrough

 

Ordering Information

P/N Description Dimension
0021887 TOYOPEARL Phenyl-600M 25 mL
0021888 TOYOPEARL Phenyl-600M 100 mL
0021889 TOYOPEARL Phenyl-600M 1 L
0021890 TOYOPEARL Phenyl-600M 5 L
0045031 ToyoScreen RoboColumn Phenyl-600M 8 x 200 µL
0045032 ToyoScreen RoboColumn Phenyl-600M 8 x 600 µL
0045123 MiniChrom TOYOPEARL Phenyl-600M, 5 mL 8 mm ID x 100 mm L
0021892 ToyoScreen Phenyl-600M 6 x 1 mL
0021893 ToyoScreen Phenyl-600M 6 x 5 mL
0021398 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 1 mL
0021399 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 5 mL