SOLUTIONS

TOYOPEARL Hexyl-650

TOYOPEARL Hexyl-650

TOYOPEARL Hexyl-650 is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with C6 groups. This resin has the highest hydrophobicity of the TOYOPEARL HIC resins. It is used for very hydrophilic proteins or where a low salt elution environment is required. It is useful as an early capture step or for intermediate purification.

TOYOPEARL Hexyl-650 is available in three particle sizes, S-, M-, and C-grade.

 

Structure of TOYOPEARL Hexyl-650 resins 

Hexyl_structure.png 

 

Product attributes

Pore size (mean): 100 nm
Particle size (mean): 100 µm (C-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Typical dynamic binding capacities for humanized mAb

HIC_fig2.png 
                                                Binding capacitiy (mg/mL - gel)
                                                (10% breakthrough)
Phenyl-650M                                           35.2
Butyl-650M                                             42.1
PPG-600M                                              44.8
Butyl-600M                                             48.5 

Selectivity of TOYOPEARL HIC resins

HIC_fig3.png
Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen

 

Ordering Information

P/N Description Dimension
0044465 TOYOPEARL Hexyl-650C 25 mL
0019026 TOYOPEARL Hexyl-650C 100 mL
0019027 TOYOPEARL Hexyl-650C 1 L
0019028 TOYOPEARL Hexyl-650C 5 L
0021378 ToyoScreen Hexyl-650C 6 x 1 mL
0021379 ToyoScreen Hexyl-650C 6 x 5 mL
0021398 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 1 mL
0021399 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 5 mL