SOLUTIONS

TOYOPEARL Ether-650M

TOYOPEARL Ether-650

TOYOPEARL Ether-650 is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with ether groups. TSKgel TOYOPEARL Ether-650 is the least hydrophobic TOYOPEARL resin and the best choice for the separation of very hydrophobic proteins or monoclonal antibodies.

TOYOPEARL Ether-650 is available in three particle sizes, 35 µm (S-grade), 65 µm (M-grade), and 100 µm (C-grade).

Structure of TOYOPEARL Ether-650 resins 

Ether_structure.png 

 

Product attributes

Pore size (mean): 100 nm
Particle size (mean): 65 µm (M-grade)
35 µm (S-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Typical dynamic binding capacities for humanized mAb

HIC_fig2.png 
                                                Binding capacitiy (mg/mL - gel)
                                                (10% breakthrough)
Phenyl-650M                                           35.2
Butyl-650M                                             42.1
PPG-600M                                              44.8
Butyl-600M                                             48.5 

Selectivity of TOYOPEARL HIC resins

HIC_fig3.png
Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen

 

Ordering Information

P/N Description Dimension
0043151 TOYOPEARL Ether-650S 25 mL
0016172 TOYOPEARL Ether-650S 100 mL
0016174 TOYOPEARL Ether-650S 1 L
0016176 TOYOPEARL Ether-650S 5 L
0019805 TOYOPEARL Ether-650M 25 mL
0016173 TOYOPEARL Ether-650M 100 mL
0016175 TOYOPEARL Ether-650M 1 L
0016177 TOYOPEARL Ether-650M 5 L
0021372 ToyoScreen Ether-650M 6 x 1 mL
0021373 ToyoScreen Ether-650M 6 x 5 mL