SOLUTIONS

TOYOPEARL Butyl-650

TOYOPEARL Butyl-650

TOYOPEARL Butyl-650 is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with C4 groups. This resin has the second highest hydrophobicity of the TOYOPEARL HIC resins. Its pore size is the largest of our three butyl resins. Primary applications are the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps.

TOYOPEARL Butyl-650 is available in three particle sizes, 35 µm (S-grade), 65 µm (M-grade), and 100 µm (C-grade).

 

Structure of TOYOPEARL Butyl-650 resins 

Butyl650_structure.png
 

 

Product attributes

Pore size (mean): 100 nm
Particle size (mean): 100 µm (C-grade)
  65 µm (M-grade)
  35 µm (M-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Typical dynamic binding capacities for humanized mAb

HIC_fig2.png 
                                                Binding capacitiy (mg/mL - gel)
                                                (10% breakthrough)
Phenyl-650M                                           35.2
Butyl-650M                                             42.1
PPG-600M                                              44.8
Butyl-600M                                             48.5 

Selectivity of TOYOPEARL HIC resins

HIC_fig3.png
Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen

 

Ordering Information

P/N Description Dimension
0043153 TOYOPEARL Butyl-650S 25 mL
0007476 TOYOPEARL Butyl-650S 100 mL
0014701 TOYOPEARL Butyl-650S 1 L
0007975 TOYOPEARL Butyl-650S 5 L
0019802 TOYOPEARL Butyl-650M 25 mL
0007477 TOYOPEARL Butyl-650M 100 mL
0014702 TOYOPEARL Butyl-650M 1 L
0007976 TOYOPEARL Butyl-650M 5 L
0043127 TOYOPEARL Butyl-650C 25 mL
0007478 TOYOPEARL Butyl-650C 100 mL
0014703 TOYOPEARL Butyl-650C 1 L
0007977 TOYOPEARL Butyl-650C 5 L
0045125 MiniChrom TOYOPEARL Butyl-650M, 5 mL 8 mm ID x 100 mm L
0045126 MiniChrom TOYOPEARL Butyl-650S, 5 mL 8 mm ID x 100 mm L
0021376 ToyoScreen Butyl-650M 6 x 5 mL
0021377 ToyoScreen Butyl-650M 6 x 1 mL
0021398 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 1 mL
0021399 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 5 mL