SOLUTIONS

TOYOPEARL Butyl-600M

TOYOPEARL Butyl-600M

TOYOPEARL Butyl-600M is based on a 75 nm (750 Å) pore size polymethacrylate base material bonded with C4 groups. It has the second highest hydrophobicity of the TOYOPEARL HIC resins. Its pore size has been optimized for the purification of monoclonal antibodies and is between that of TOYOPEARLl Butyl-650 and TOYOPEARL SuperButyl-550 media.

TOYOPEARL Butyl-600M is typically used for intermediate purification or polishing steps.

 

Structure of TOYOPEARL Butyl-600 resins 

Butyl600_structure.png
 

 

Product attributes

Pore size (mean): 75 nm
Particle size (mean): 65 µm (M-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Comparison of TOYOPEARL -600M resins

HIC_600resins_fig1.png
 
Resins:            A. TOYOPEARL PPG-600M
                         B. TOYOPEARL Phenyl-600M
                         C. TOYOPEARL Butyl-600M
Column size:  7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO4 + 0.1 mol/L sodium phosphate, pH 7.0
                         Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient:        60 min linear gradient from buffer A to B
Flow rate:       136 cm/hr (1.0 mL/min)
Detection:       UV @ 280 nm
Temperature: ambient
Samples:        1 g/L of: 1. ribonuclease A, 2.lysozyme, 3. α-chymotrypsinogen
Load volume: 100 µL

Breakthrough curves of polyclonal IgG on various HIC resins

HIC_600resins_fig2.png

Resins:           A. TOYOPEARL PPG-600M
                        B. TOYOPEARL Phenyl-650M
                        C. TOYOPEARL Butyl-600M
                        D. Competitor Phenyl Agarose
                        E. TOYOPEARL Phenyl-600M
Column size:  7.8 mm ID × 20 cm
Mobile phase: 0.1 mol/L sodium phosphate, pH 7.0 + 0.8 mol/L (NH4)2SO4
Flow rate:       300 cm/hr (2.4 mL/min)
Detection:       UV @ 280 nm
Temperature: 25 °C
Samples:         1.0 g/L polyclonal IgG

DBC was calculated at 10% breakthrough

 

Ordering Information

P/N Description Dimension
0021448 TOYOPEARL Butyl-600M 25 mL
0021449 TOYOPEARL Butyl-600M 100 mL
0021450 TOYOPEARL Butyl-600M 1 L
0021451 TOYOPEARL Butyl-600M 5 L
0045127 MiniChrom TOYOPEARL Butyl-600M, 5 mL 8 mm ID x 100 mm L
0045033 ToyoScreen RoboColumn Butyl-600M 8 x 200 µL
0045034 ToyoScreen RoboColumn Butyl-600M 8 x 600 µL
0021494 ToyoScreen Butyl-600M 6 x 1 mL
0021495 ToyoScreen Butyl-600M 6 x 5 mL
0021398 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 1 mL
0021399 ToyoScreen HIC (PPG-600M, Butyl-600M,
Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)
6 x 5 mL