SOLUTIONS

Biopharmaceutical SEC Applications

Biopharmaceutical UHP-SEC Applications using TSKgel UP-SW3000

In a supplement of LCGC North America, Genentech researchers described the use of UHP-SEC for the SEC analysis of complex and novel biotherapeutic products and PEG-protein conjugates.  

The authors describe various examples of using size exclusion chromatography for novel biotherapeutics at Genentech. These cover antibodies, antibody-drug-conjugates, bispecific mAbs and coformulations of mAbs and Fab. The advantage of using UHPLC is shown by comparing UHPLC and HPLC results.  

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Conjugation of poly (ethylene glycol), PEG, to therapeutic molecules is widely used to increase half-life of therapeutics. This article is discussing the specific requirements when analyzing PEG-protein conjugates.

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