SOLUTIONS

TSKgel UP-SW2000

TSKgel UP-SW2000

TSKgel UP-SW2000 columns are designed for the analysis of small proteins, peptides and oligonucleotides. The columns are packed with 2 μm silica-based beads shielded with a hydrophilic diol-type bonded phase that prevents the silica surface from interacting with protein samples.

TSKgel UP-SW2000 columns feature the same pore size as the well-established TSKgel SuperSW2000 columns. Hence methods developed using TSKgel SuperSW2000 columns can easily be transferred to TSKgel UP-SW2000 columns on conventional HPLC systems as well as on UHPLC systems.

TSKgel UP-SW2000 columns offer higher resolution, improved peak shape and increased efficiency yielding methods that are robust, reproducible and easily transferrable between UHPLC and HPLC systems.

TSKgel UP-SW2000 columns offer superior reproducibility injection-to-injection, from column-to-column within the same lot and from lot-to-lot. %RSD values for peak parameters including retention times, area peak height, peak asymmetry, and theoretical plate count demonstrate the exceptional reproducibility of these columns.

Analysis of Oligonucleotides

SEC_UP-SW2000_fig1App.pngSEC_UP-SW2000 - Oligonucleotides
Column: TSKgel UP-SW2000, 2 µm, 
4.6 mm ID × 30 cm × 2
Mobile phase: 0.05% NaN3 and 0.3 mol/L NaCl in 0.05 mol/L phosphate, pH 6.7
Flow rate: 0.2 mL/min
Detection: UV @ 260 nm
Temperature: 25 °C
Sample: 20 mer: 5’-GAATTCATCGGTTCAGAGAC-3’
19 mer: 5’-AATTCATCGGTTCAGAGAC-3’

Analysis of Small Protein and Peptide Mixture

SEC_UP-SW2000 Protein and Peptide Mixture
Column: TSKgel UP-SW2000, 2 µm, 
4.6 mm ID × 30 cm
Mobile phase: 100 mmol/L phosphate buffer, pH 6.7, 
100 mmol/L Na2SO4, 0.05% NaN3
Flow rate: 0.35 mL/min
Instruments: Agilent 1100 with Chemstation v. 2.18.18
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 5 µL
Samples: Peptide 1: Myoglobin 16.7 kDa
Peptide 2: RPDFC LEPPY TGPCK ARIIR YFYNA KAGLC QTFVY GGCRA KRNNF KSAED CMRTC GGA (58 aa) 6.5 kDa
Peptide 3: QRLGNQWAVGHLM (14 aa) 
1.6 kDa

Product Attributes

Base material: silica
Particle size (mean): 2 µm
Pore size (mean): 12.5 nm
Functional group: Diol
pH stability: 2.5 - 7.5
Calibration range (proteins): 1,000 - 1.5 ×105 Da (globular proteins)

 

Ordering Information

P/N Description Dimension
0023515 TSKgel UP-SW2000 4.6 mm ID x 15 cm L
0023514 TSKgel UP-SW2000 4.6 mm ID x 30 cm L
0023516 TSKgel Guardcolumn UP-SW2000 4.6 mm ID x 2.0 cm L
0023517 TSKgel DC Guardcolumn UP-SW2000 4.6 mm ID x 2.0 cm L