SOLUTIONS

TSKgel G3000SWXL

TSKgel G3000SWXL

Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel G3000SWXL, 5 μm columns are an excellent choice for protein and large peptide separations. The fractionation range is applicable for globular protein samples up to 500 kDa. The typical application is the separation of IgG fragments, monomers and aggregates. These columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.

Also available within the TSKgel G3000SWXL column line is a BioAssist column made of PEEK housing material to reduce sample adsorption to stainless steel or glass, and QC-PAK columns, which are 15 cm in length with 5 µm particles and offer the same resolution in half the time as the 30 cm TSKgel G3000SWXL column.

Size variant analysis of conjugates using a TSKgel G3000SWXL column with mobile phase 0.2 mol/L KPi and 0.25 mol/L KCl (A) and 85% KPi/KCl, 15% 2-propanol (B)

sw3000swxl_fig1.png
Column:          TSKgel G3000SWXL, 7.8 mm ID × 30 cm
Mobile phase: A: 0.2 mol/L KPi and 0.25 mol/L KCl, pH 6.95
                         B: 85% KPi/KCl + 15% 2-propanol
Flow rate:        0.5 mL/min
Detection:       UV @ 280 nm

Republished with permission of Genentech, Inc., from Analytical methods for physicochemical characterization of antibody drug conjugates, Wakankar A, Chen Y, Gokarn Y, Jacobson FS., 3, 2011; permission conveyed through Copyright Clearance Center, Inc.

SEC analysis of reaction mixtures

sw3000swxl_fig2.png 
Column:          TSKgel G3000SWXL, 5 μm, 7.8 mm ID × 30 cm
Mobile phase: 0.1 mol/L phosphate buffer, 0.1 mol/L Na2SO4, pH 6.7
Flow rate:       1.0 mL/min
Detection:       UV @ 280 nm
Injection vol.: 20 μL
Sample:           5, 10, 30 kDa methoxy PEG aldehyde 

 

Ordering Information

P/N Description Dimension
0008541 TSKgel G3000SWXL 7.8 mm ID x 30 cm L
0008543 TSKgel SWXL Guardcolumn 6.0 mm ID x 4 cm L
0016049  TSKgel QC-PAK GFC 300  7.8 mm ID x 30 cm L 
0020026 TSKgel BioAssist G3SWXL, PEEK 7.8 mm ID x 30 cm L 
0018008 TSKgel BioAssist Guardcolumn  6.0 mm ID x 4 cm L