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TSKgel SuperSW mAb HR

TSKgel SuperSW mAb HR

The TSKgel SuperSW mAb HR gel filtration column offers high resolution analysis of mAb fragments, monomer, and dimer. The high resolution capability of this column is due to a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody.

Separation of human IgG monomer, half-body, and fragments using a TSKgel SuperSW mAb HR column

superswmab_hr_fig1.png 
Column:          TSKgel SuperSW mAb HR, 4 μm, 7.8 mm ID × 30 cm
Mobile phase:  0.1 mol/L phosphate/0.1 mol/L sulfate buffer + 0.05% NaN3
Flow rate:         0.5 mL/min
Detection:        UV @ 280 nm
Temperature:  25 ºC
Injection vol.:  10 μL
Sample:            human IgG (4.6 g/L) from Sigma Aldrich 

 

Comparison of resolution of mAb monomer and dimer

superswmab_hr_fig2.png 
Columns:         A. TSKgel SuperSW mAb HR, 4 µm, 7.8 mm ID × 30 cm
                          B. Brand A, 3 μm, 7.8 mm ID × 30 cm
                          C. Brand B, 5 μm, 7.8 mm ID × 30 cm
                          D. Brand C, 5 μm, 8.0 mm ID × 30 cm
                          E. Brand D, 5 μm, 8.0 mm ID × 30 cm
Mobile phase:  200 mmol/L phosphate buffer, pH 6.7 + 0.05% NaN3
Flow rate:        1.0 mL/min
Detection:        UV @ 280 nm
Temperature:  25 ˚C
Injection vol.: 10 μL
Sample:           IgG (human polyclonal), 1.0 g/L

 

High durability of TSKgel SuperSW mAb HR column

superswmab_hr_fig3.png 
Column:          TSKgel SuperSW mAb HR, 4 µm, 7.8 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.7 + 0.05% NaN3
Flow rate:        0.8 mL/min
Detection:       UV @ 280 nm
Injection vol.: 10 μL
Samples:         1. g-Globulin
                         2. Cytochrome C
                         3. DNP-L-Alanine 

 

Ordering Information

P/N Description Dimension
0022854 TSKgel SuperSW mAb HR 7.8 mm ID x 30 cm L
0022857 TSKgel Guardcolumn SuperSW mAb 6.0 mm ID x 4.0 cm L