EcoSEC GPC System

EcoSEC GPC/SEC System 

EcoSEC Ambient Instrument


The EcoSEC GPC System is a dedicated GPC instrument developed for fast polymer analysis. The all-in-one design concept delivers these benefits: 
Low dead volume for improved resolution and molar mass distribution accuracy 
Temperature controlled pump heads for excellent flow rate precision regardless of changes in laboratory temperature 
Dual flow RI (refractive index) detection for unmatched baseline stability 


Time and solvent can be saved using the EcoSEC GPC System with optional semi-micro columns due to the system’s low dead volume. The dead volume of the EcoSEC GPC System (<20 μL) is less than half the dead volume of conventional GPC systems.

What sets an EcoSEC GPC System apart? 
Comprehensive temperature control for retention times which are independent of temperature fluctuations in the lab »
Dual flow RI design for unmatched baseline stability »
Best in class retention time precision for accurate molar mass measurement and proven site-to-site comparability »
Low dead volume design for improved resolution, can save up to 50% of analysis time and up to 80% solvent consumption when using 4.6 mm ID × 15 cm GPC columns »
Wide range of GPC columns »



Click on the individual component links below for more detailed information.


Control Panel »
Autosampler »
Pumps »
RI Detector »
Column Oven »
UV Detector Option »
Column Switching Valve Option »


Tosoh Bioscience can tailor a system to meet your application needs.

Does your analysis require additional detectors beyond RI and UV?
The EcoSEC GPC System provides easy and effortless connectivity when using multi-detector configurations. We offer external light scattering and viscometry detectors.

Contact us for a quote!

Dimensions 680 (W) × 500 (D) × 580 (H) mm
Dead volume < 20 µL


Injection volume 1 to 1,500 µL in 1 µL increments <20 µL
Number of samples < 100


Flow rate 10 to 2,000 µL/min in 1 µL/min steps
Accuracy < +/- 2%
Precision < +/- 0.2%
Max. pressure < 25 MPA or 3,500 psi


Column Oven
Temperature range ambient plus 10 °C to 60 °C
Capacity 7.8 mm ID × 30 cm × 8 columns
Precision < +/- 0.2%
Max. pressure < 25 MPA or 3,500 psi


RI Detector
Type Bryce or dual flow type. Tungsten light source (1.00 - 1.80 RI range)
Cell volume 2.5 µL
Noise 2 × 10-9 RI units (RIU)
Drift 1× 10-7 RIU/h (THF, 1.0 mL/min)


UV Detector (optional)
Flow rate 195 to 350 nm
Cell volume 2.5 µL
Time constant 0.5, 1.0 and 3.0 seconds
Noise  2.5× 10-5 AU (254 nm) 
Drift 3× 10-4 AU/h (254 nm)

The EcoSEC GPC System Workstation Software offers unparalleled versatility and includes these features:
GPC-specific EcoSEC GPC System software to simplify system control and data handling
Controls up to 2 EcoSEC GPC Systems
Excellent data handling and report generation
Fully featured data handling system; analyze data from two detectors
Start and stop system automatically
Column Switching Valve Option
One license for multiple locations


Software Features include: 
Flow Diagram »
Method Templates »
Peak Editing »
Data Management »
Multiprocessing Function »
GPC Specific Quantitative Calculations »
Report Generation »
Software Specifications »



PStQuick polystyrene calibration standards

PStQuick polystyrene calibration standards contain pre-mixed quantities of polystyrene polymers in autosampler vials for the calibration of GPC columns. Addition of solvent is all that is required for easy preparation and analysis. 12 different kits containing polystyrene polymers of various molar masses are available. Of the 12 kits, 9 are individual kits, each containing 3 to 5 polystyrene polymers. The remaining 3 are composite kits containing 2 or 3 of the individual kits.

PStQuick MP kits for calibrating TSKgel SuperMultiporeHZ columns  

  • PStQuick MP-N for TSKgel SuperMultiporeHZ-N
  • PStQuick MP-M for TSKgel SuperMultiporeHZ-M
  • PStQuick MP-H for TSKgel SuperMultiporeHZ-H


PStQuick kits to calibrate other TSKgel GPC columns and GPC columns from other manufacturers

  • PStQuick Kit-L for calibrating TSKgel mixed-bed N-type columns
  • PStQuick Kit-M for calibrating TSKgel mixed-bed M-type columns
  • PStQuick Kit-H for calibrating TSKgel mixed-bed H-type columns

The concentrations of the polystyrene standards in the premixed vials vary from a low of 0.2mg/mL to a high of 1.6mg/mL.

All PStQuick polystyrene calibration standards are available for reordering.

Contents of each kit
For example, PStQuick Kit-M contains 20 vials each of grades C and D.

Calibration Mixtures
A B C D E F G H Vials 
PStQuick MP-N SuperMultipore
        X 60
PStQuick MP-M SuperMultipore
            X   60
PStQuick MP-H SuperMultipore
  X             60
PStQuick Kit -H
(High MW)
Mixed Bed
X X X           60
(3 x 20)
PStQuick Kit -M
(Medium MW)
Mixed Bed
    X X         40
(2 x 20)
PStQuick Kit -L
(Low MW)
Mixed Bed
        X X     40
(2 x 20)

Nominal MW of Kit Components

For example, grade B contains polystyrene polymers of nominal molecular weights 5,480,000 - 706,000, 96,400 - 10,200 and 1,000. In the above Table it is shown that grade B is part of PStQuick MP-H and PStQuick Kit-H.

Polystyrene MW A B C D E F G H
8,420,000 X
5,480,000 X
2,890,000 X
1,090,000 X X
706,000 X X
355,000 X X
190,000 X X X
96,400 X X
37,900 X X X
18,100 X X X
10,200 X
5,970 X X X X
2,500 X X X
1,000 X X
500 X X X X

Procedure - Simply add solvent, mix and inject onto the GPC column.

Tosoh introduced its first line of TSKgel GPC columns in 1971. Ever since, Tosoh scientists have made important contributions to advances in polymer analysis by developing state-of-the-art GPC columns for the most demanding applications.

Semi-micro columns are the TSKgel columns of choice for use with the EcoSEC GPC System. They are referred to as such since their dimensions are smaller than conventional columns in terms of internal diameter as well as in length: 4.6 mm or 6 mm ID × 15 cm vs. 7.8 mm ID × 30 cm.