TSKgel UP-SW2000 columns are designed for the analysis of small proteins, peptides and oligonucleotides. The columns are packed with 2 μm silica-based beads shielded with a hydrophilic diol-type bonded phase that prevents the silica surface from interacting with protein samples.
TSKgel UP-SW2000 columns feature the same pore size as the well-established TSKgel SuperSW2000 columns. Hence methods developed using TSKgel SuperSW2000 columns can easily be transferred to TSKgel UP-SW2000 columns on conventional HPLC systems as well as on UHPLC systems.
TSKgel UP-SW2000 columns offer higher resolution, improved peak shape and increased efficiency yielding methods that are robust, reproducible and easily transferrable between UHPLC and HPLC systems.
TSKgel UP-SW2000 columns offer superior reproducibility injection-to-injection, from column-to-column within the same lot and from lot-to-lot. %RSD values for peak parameters including retention times, area peak height, peak asymmetry, and theoretical plate count demonstrate the exceptional reproducibility of these columns.
Analysis of Oligonucleotides |
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| Column: | TSKgel UP-SW2000, 2 µm,
4.6 mm ID × 30 cm × 2 | | Mobile phase: | 0.05% NaN3 and 0.3 mol/L NaCl in 0.05 mol/L phosphate, pH 6.7 | | Flow rate: | 0.2 mL/min | | Detection: | UV @ 260 nm | | Temperature: | 25 °C | | Sample: | 20 mer: 5’-GAATTCATCGGTTCAGAGAC-3’
19 mer: 5’-AATTCATCGGTTCAGAGAC-3’ | |
Analysis of Small Protein and Peptide Mixture |
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| Column: | TSKgel UP-SW2000, 2 µm,
4.6 mm ID × 30 cm | | Mobile phase: | 100 mmol/L phosphate buffer, pH 6.7,
100 mmol/L Na2SO4, 0.05% NaN3 | | Flow rate: | 0.35 mL/min | | Instruments: | Agilent 1100 with Chemstation v. 2.18.18 | | Detection: | UV @ 280 nm | | Temperature: | 25 °C | | Injection vol.: | 5 µL | | Samples: | Peptide 1: Myoglobin 16.7 kDa
Peptide 2: RPDFC LEPPY TGPCK ARIIR YFYNA KAGLC QTFVY GGCRA KRNNF KSAED CMRTC GGA (58 aa) 6.5 kDa
Peptide 3: QRLGNQWAVGHLM (14 aa)
1.6 kDa | |