Bioseparation Forum

Virtual BioSeparation Forum  

Based on the track record of the BioSeparation Forum events we created a virtual event packed with inspiring presentations, scientific updates and opportunities to connect with your peers. The virtual BioSeparation Forum series presents talks on the use of bio-chromatography for manufacturing and analysis of specific target molecules.

Gain insights into latest research projects related to SARS-CoV2 /COVID-19

The next virtual BioSeparation Forum on October 21, 2021 will focus on on Covid-19 related research.

Attend inspiring presentations and scientific updates presented by experts form biopharma, academia, and Tosoh Bioscience. 

Program: 


COR-101: Development of a human recombinant antibody for the treatment of COVID-19 
Michael Hust, TU Braunschweig, Germany

COR-101 is a fully human, Fc silenced IgG that was discovered by antibody phage display. It reduced the SARS-CoV-2 virus load in the lung by more than 99 percent in Hamster models and led to much faster recovery. Its mode of action has been elucidated by solving the atomic structure of its interaction with SARS-CoV-2. The antibody competes with ACE2 binding by blocking a large area of the SARS-CoV-2 spike protein.


SARS-CoV-2: CHO-based manufacturing provides high quality subunit-vaccine candidates and diagnostics
Paco Pino, ExellGene SA, Monthey, Switzerland

The Spike protein of SARS-COV-2 is a typical virus surface antigen. The recent COVID19 pandemic showed that contemporary protein production technologies for such subunit protein antigens are insufficient to meet the unprecedented global demand for critical ingredients. Using optimized Chinese Hamster Ovary based technologies, we developed a scalable, GMP-compliant, chemically defined processes for production of such complex proteins. In weeks-timeframes we were able to produce grams quantities of fully active SARS-COV-2 Spike protein trimers. The spike trimer resulted in high neutralizing antibody titers against SARS-COV-2 in several animal models and showed extreme sensitivity and specificity in diagnostic tests.


Using SEC-MALS in the analysis of SARS-CoV-2 spike protein and vaccine delivery systems
Maurice Diwo, Tosoh Bioscience GmbH, Griesheim, Germany

Multi-Angle Light Scattering (MALS) detection combined with Size Exclusion Chromatography (SEC) is a popular approach to measure molecular weight (MW) and size (radius of gyration, Rg) of molecular complexes and macromolecules. Using Tosoh’s recently launched LenS3 MALS detector we analyzed the recombinant spike protein of SARS-CoV-2, developed by ExcellGene SA in order to validate its quality. The product can be used for diagnostic tests, vaccine production and monoclonal antibody (mAb) generation. SEC-MALS is also widely used in the analysis of modern delivery vehicles for nucleic acids such as lipid nanoparticles or virus-like particles (VLPs). Here, we present latest data on VLP analysis by SEC-MALS using the new LenS3 MALS detector.


Joint Question & Answer Session with all presenters 


Register here!


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Bioforum October