The TOYOPEARL GigaCap family consists of two cation exchange
resins – GigaCap S-650 and GigaCap CM-650 – and the GigaCap
Q-650 and DEAE-650 anion exchange resins. While all GigaCap
media are available in M-grade particle size (75 μm) for capture and
intermediate process steps, GigaCap S and Q are now also available
in smaller particle size (S-grade; 35 μm) for high resolution inter-
mediate and polishing purification.
TOYOPEARL GigaCap S-650S
is a strong cation exchange resin
that combines advantageous pressure flow characteristics with
excellent dynamic binding capacities (DBC) and high recoveries for
a wide range of biomolecules. TOYOPEARL GigaCap S-650S offers a
typical DBC of up to 165 g human IgG/L resin and a higher resolution
than the 75 µm M-grade. It is ideally suited for high purity separations
needed in final polishing steps. Good mass transfer kinetics enable the
resin to maintain its dynamic binding capacity (DBC) at higher linear
velocities. The figure below shows the high resolution achieved
with TOYOPEARL GigaCap S-650S and its advantageous selectivity,
when compared with agarose based cation exchange media with
similar particle size.
TOYOPEARL GigaCap Q-650S
is a strong anion exchange resin
that offers DBCs approaching 190 g/L for BSA. This resin is ideal
for separations that need a high resolution, such as the purification
of oligonucleotides. The purification of oligonucleotides by anion
exchange chromatography has traditionally fallen to resins such as
TSKgel SuperQ-5PW (20) that offer high resolution and selectivity
in conjunction with excellent mechanical stability at high column
pressures. TOYOPEARL GigaCap Q-650S offers a low pressure
alternative while preserving the selectivity, resolution and yields of
those higher pressure processes. The figures below show that the
N-1 peak was slightly better resolved with TSKgel SuperQ-5PW (20)
than with TOYOPEARL GigaCap Q-650S. HPLC analysis of fractions
taken across the peaks revealed that both resins were able to
adequately resolve the full length oligonucleotide.
Tosoh
customer magazine
ADDITIONS TO THE TOYOPEARL GigaCap FAMILY
03
what‘s new
resins
The development of high expression cell lines has triggered the demand for high throughput downstream
processing. This requires rigid resins that offer high capacities and excellent recoveries at high flow rates.
The renowned TOYOPEARL GigaCap ion exchange resins meet these needs and are ideally suited for purification of
monoclonal antibodies and other biomolecules. Recently TOYOPEARL GigaCap DEAE was introduced to expand the
choice of available functionalities by a weak anion exchange resin. Customer demands for smaller particle size
versions of the existing resins initiated the development of TOYOPEARL GigaCap S-650S and Q-650S.
Purification of Oligonucleotides on TOYOPEARL GigaCap Q-650S
Selectivity of small particle size cation exchange resins
Purification of Oligonucleotides on TSKgel SuperQ-5PW (20)
elution volume [mL]
detector response [mAu]
concentration b-buffer [%]
0
150
200
100
50
-15
35
85
135
185
235
285
335
385
435
100
50
0
N-1
elution volume [mL]
detector response [mAu]
concentration b-buffer [%]
0
150
200
100
50
80
180
280
380
480
580
680
780
100
50
0
N-1
time [min]
[mAu]
5
25
35
30
15
20
10
0
20
40
60
80
100
120
140
Capto S ImpRes
3
2
1
SP Sepharose HP
TOYOPEARL GigaCap S-650S
