TOSOH
CUSTOMER MAGAZINE
Protein L based affinity chromatography is used for the capture of antibo-
dies and antibody fragments that do not bind to Protein A. Unlike Protein
A and G, which bind to the Fc region of immunoglobulins (IgGs), Protein
L binds through interaction with the variable region of an antibody’s kappa
light chain. Therefore Protein L binds a wider range of antibody classes
than Protein A. Figure 1 shows typical targets, such as antigen binding
fragments (Fabs), single-chain variable fragments (scFvs) and domain anti-
bodies (dAbs). Protein L also helps to purify those immunoglobulins who-
se Fc region is not easily accessible for Protein A, such as IgM or IgA.
TOYOPEARL AF-rProtein L-650F is an affinity chromatography resin that
combines a rigid polymer matrix with a recombinant ligand, which is ex-
pressed in E.coli. Code optimization results in higher binding capacity and
an improved stability of the ligand compared to the native Protein L mole-
cule. The new resin provides the highest binding capacity available on the
market for Fab molecules.
Figure 2 shows the binding capacity of TOYOPEARL AF-rProtein L-650F
for a Fab fragment at various residence times in comparison to the most
popular commercially available agarose based Protein L medium. Due to
the excellent mass transfer characteristics of the new resin, especially
dynamic binding capacities at 1 to 3 minutes residence time excel those
obtained with the agarose-based resin. As affinity resin costs represent a
considerable part of the overall production costs the high binding capacity
of the new protein L resin can help to improve process economics in the
production of antibody related recombinant molecules.
“TOYOPEARL AF-RPROTEINL-650F IS AN INNOVATIVE AND VERY USEFUL
CHROMATOGRAPHIC RESIN IN MY PURIFICATION TOOLBOX, AS IT ALLOWS CAP-
TURE OF MULTIPLE ANTIBODY TYPES. IT IS THE RESIN I’VE BEEN EXPECTING FOR
MANY YEARS.”
DR. MICHAEL DAVIDS - CEO DAVIDS BIOTECHNOLOGIE GMBH
03
WHAT’S NEW
RESINS
TOYOPEARL PROTEIN L RESIN FOR THE PURIFICATION
OF ANTIBODY FRAGMENTS
A HIGH CAPACITY PROTEIN L RESIN COMPLEMENTS THE RANGE OF TOYOPEARL MEDIA FOR ANTIBODY PURIFICATION AND MANUFAC-
TURING. THE NEW PROTEIN L AFFINITY RESIN, TOYOPEARL® AF-R PROTEIN L-650F, COMBINES THE RIGID TOYOPEARL POLYMER MATRIX
WITH AN ENGINEERED RECOMBINANT PROTEIN L DERIVED LIGAND. IT ENABLES AN EASY PURIFICATION OF ANTIBODY RELATED TAR-
GETS THAT CANNOT BE PURIFIED BY PROTEIN A CHROMATOGRAPHY.
FIGURE 1:
TARGETS FOR PROTEIN L AFFINITY CAPTURING
FIGURE 2:
BINDING CAPACITY OF PROTEIN L RESINS
0
4
6
8
10
2
Retention Time (min)
TOYOPEARL AF-rProtein L-650F
Agarose based Protein L
60
50
40
30
20
10
0
DBC for Fab [mg/mL-resin]
Fv
Fab
Heavy
chain
Light
chain
Fc
mAb (150 kDa)
Fab (55 kDa)
scFv (25 kDa)
