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Iec
IEC
67
Performance enhancement on FPLC system
BioAssist Q
Competitor Q
10
Minutes
5
15
mAU
mAU
1200
1180
1600
1140
1120
1100
1080
1060
1040
1200
1180
1600
1140
1120
1100
1080
1060
1040
0
Column:
TSKgel BioAssist Q, 4.6 mm ID x 5 cm (PEEK)
Competitor Q, 5.0 mm ID x 5 cm
Elution:
30 min linear gradient from 0 to 1 mol/L NaCl
in 20 mmol/L sodium phosphate pH 8.0
Flow Rate:
1.0 mL/min
Detection:
UV @ 280 nm
Sample:
1) conalbumin, i) ovalbumin impurity
2) ovalbumin, 3) trypsin inhibitor
1
i
2
3
1
i
2
3
Polymer-based Anion Exchange Columns
TSKgel BioAssist Q is suitable for use in systems that are designed for
laboratory or semi-preparative applications.
Figure 3
demonstrates the
performance enhancement of TSKgel BioAssist Q over a competitive
product when operated side-by-side on an FPLC system.
TABLE II
shows typical dynamic binding capacities on BioAssist Q relative to
competitive products.
Column: TSKgel BioAssist Q, 4.6 mm ID x 5 cm L (PEEK),
Competitor Q, 5.0 mm ID x 5 cm L; Elution: 30 min linear gradient from 0
to 1 mol/L NaCl in 20 mmol/L sodium phosphate pH 8.0; Flow Rate: 1.0 mL/
min; Detection: UV@280nm; Sample: 1) conalbumin, i) ovalbumin impurity,
2) ovalbumin, 3) trypsin inhibitor
figure 3
Performance enhancement on FPLC system
Binding capacity (mg/mL)
BioAssist Q
SuperQ
Conv.
Conv.
-5PW
Q type
Q type
Protein
prod. A
prod. B
Thyroglobulin
77.4
22.9
20.2
1.8
Monoclonal IgG
1
57.8
43.3
46.7
47.7
Human Serum
83.1
78.9
48.2
48.8
Albumin
Trypsin Inhibitor
84.3
92.8
51.8
57.8
Columns:
TSKgel BioAssist Q (4.6 mmID x 1 cm L)
TSKgel SuperQ-5PW (4.6 mmID x 1 cm L)
Conventional Q type product A (4.6 mm ID x 1 cm L)
Conventional Q type product B (4.6 mm ID x 1 cm L)
Solvent:
20 mmol/L Tris-HCl buffer, pH 8.0
Flow rate:
0.38 mL/min
Detection:
UV@280nm)
*The capacity was determined at 10% height of the breakthrough
curve at UV 280 nm.
TABLE I
Basic Properties of TSKgel STAT Anion Exchange Columns
Applications of TSKgel ANION exchange columns
Property
TSKgel Q-STAT
TSKgel DNA-STAT
Base material
Cross-linked hydrophilic polymer (mono-disperse particles)
Pore size
Non-porous
Functional group
Quaternary ammonium (same chemistry)
Particle size
7 µm
10 µm
5 µm
Column size
4.6 mm ID x 10 cm L
3 mm ID x 3.5 cm L
4.6 mm ID x 10 cm L
Application
High resolution
protein separation
High resolution
protein separation
High resolution
DNA separations
TABLE II
Comparison of dynamic binding capacities