Basic HTML Version
tosoh bioscience
chromatography catalog
3
tosoh bioscience
chromatography
catalog
TSKgel columns
TSKgel columns are known worldwide for their reliability and suitability
for a variety of chromatographic applications. Applications using TSK-
gel columns are continuously published in the scientific journals and are
listed in the current U.S. Pharmacopoeia (see Appendix C). Prepacked
columns contain TSKgel media designed for the analysis of proteins,
peptides, biopolymers and low molecular weight compounds by size ex-
clusion, ion exchange, hydrophobic interaction, reversed phase, affinity
and normal phase chromatography. The packings in the columns are
either silica-based or polymeric-based material, in particle sizes rang-
ing from 2 µm to 20 µm.
Columns are available in analytical to preparative sizes, in stainless
steel, PEEK®, or glass. To ensure specified column performance and to
maximize the longevity of your Tosoh columns, please note the guide-
lines found in Appendix A for the use, cleaning, rehydration, and storage
of your TSKgel columns.
ToyoScreen Process Development columns
ToyoScreen Process Development columns are available as an addi-
tional tool for convenient scale-up. These are easy-to-use, pre-packed
columns containing Tosoh Bioscience‘s most popular TOYOPEARL res-
ins. They provide a convenient, low-cost product for the evaluation of
TOYOPEARL ligand chemistries. ToyoScreen Process Development col-
umns are available in packages of six by 1mL and six by 5mL volumes
for affinity, ion exchange and hydrophobic interaction chromatography.
TOYOPEARL and TSKgel LabPak Media
LabPak products are small package sizes of TOYOPEARL and TSKgel
bulk media products. Typically they contain three or four different ligand
types offered for a particular chromatography mode. They are useful
for developmental scientists who wish to familiarize themselves with
resin physical properties in differing buffer systems. The larger resin
amounts in LabPak products allow the packing of wider bore and longer
columns than what is available in the ToyoScreen products. This helps
the developmental scientist or engineer to measure more accurately,
under actual packing conditions, the resin‘s dynamic binding capacity,
and selectivity.
TSKgel resins
The same media used in TSKgel columns are also available in bulk. They
are offered in particle sizes of 20 µm and 30 µm, for ion exchange and
hydrophobic interaction chromatography. TSKgel media are the most
efficient packing materials available from Tosoh Bioscience for process
chromatography. In tandem with their high efficiency, high mechanical
stability and permeability, TSKgel resins are an excellent choice under
medium to high pressure conditions.
TOYOPEARL resins
TOYOPEARL resins are hydrophilic, macroporous, bulk bioprocessing
media suitable for large-scale chromatographic applications. Because
of their polymeric backbone structure, the rigid TOYOPEARL packings
assure excellent pressure/flow characteristics. The media are stable
over the pH range of 2-12 for normal operating conditions and pH 2-14
for cleaning conditions. The particle sizes are: 20-50 µm superfine grade
for the highest performance, 40-90 µm medium grade for economical
purification, and 50-150 µm coarse grade for capture chromatography.
Large pore sizes ensure greater capacity for high molecular weight mol-
ecules, while allowing faster separation and recycling times. TOYOPE-
ARL media are available for size exclusion, ion exchange, hydrophobic
interaction, and affinity chromatography.
For predictable results in scale-up, TOYOPEARL resins are based on
the same chemistry as the prepacked TSKgel columns. This allows the
seamless scale-up of methods developed on TSKgel columns to TOYO-
PEARL, without additional optimization at pilot scale. In addition, TOYO-
PEARL resins are also available in the ToyoScreen Process Develop-
ment columns for convenient scouting and methods development.