Basic HTML Version
IEC
64
TSKgel Cation Exchange Columns
TSKgel
Matrix*
Particle
Pore
Functional
Counter
Excl. limit,
Capacity
Small ion
pKa
Column
size (µm)
size (Å)
group
ion
PEG
**
(Da)
(mg/mL)
capacity
hard-
meq/mL
ware
***
BioAssist S
pMA
7, 13
~1300
Sulfopropyl
Na
+
~4,000,000
70
(1)
0.1
2.4
PEEK
SP-5PW
pMA
10, 13, 20
1000
Sulfopropyl
Na
+
1,000,000
40
(2)
> 0.1
2.3
S, G
CM-5PW
pMA
10, 13
1000
Carboxymethyl
Na
+
1,000,000
45
(2)
> 0.1
4.2
S, G
SP-STAT
pMA
7, 10
~ 0
Sulfopropyl
Na
+
500
10
(3)
> 0.023
4.0
S
CM-STAT
pMA
7, 10
~ 0
Carboxymethyl
Na
+
500
15
(3)
> 0.1
4.9
S
SP-NPR
pMA
2.5
~ 0
Sulfopropyl
Na
+
500
5
(2)
> 0.1
2.3
S
SP-2SW
Silica
5
125
Sulfopropyl
Na
+
10,000
ND
0.3
2.2
S
CM-2SW
Silica
5
125
Carboxymethyl
Na
+
10,000
110
(2)
> 0.3
4.2
S
CM-3SW
Silica
10
250
Carboxymethyl
Na
+
30,000
ND
> 0.3
4.2
S
SCX
PS-DVB
5
60
Sulfonic acid
Na
+
, H
+
ND
> 1.5
S
*
pMA = poly methacrylate; PS-DVB = polystyrene-divinylbenzene ** Polyethylene glycol
*** PEEK = polyethyletherketone, S = stainless steel, G = glass
(1)
γ
-globulin; (2) hemoglobin; (3) lysozyme
TSKgel Anion Exchange Columns
TSKgel
Matrix*
Particle
Pore
Functional
Counter
Excl. limit,
Capacity
Small ion
pKa
Column
size (µm)
size (Å)
group
ion
PEG
**
(Da) (mg BSA/mL) capacity
hard-
meq/mL
ware
***
BioAssist Q
pMA
10, 13
~4000
Polyamine
Cl -
>5,000,000
70
0.1
9.4
PEEK
SuperQ-5PW
pMA
10,13
1000
Trimethyl-amino
Cl -
1,000,000
100
> 0.13
12.2
S, G
DEAE-5PW
pMA
10,13, 20
1000
DEAE
Cl -
1,000,000
30
0.1
11.5
S, G
Q-STAT
pMA
7,10
~ 0
Trimethyl-amino
Cl -
500
20
0.27
10.5
S
DNA-STAT
pMA
5
~ 0
Trimethyl-amino
Cl -
500
35
0.27
10.5
S
DEAE-NPR
pMA
2.5
~ 0
DEAE
Cl -
500
5
> 0.1
11.2
S
DNA-NPR
pMA
2.5
~ 0
Proprietary
ClO
4
-
500
5
> 0.1
11.2
S
DEAE-2SW
Silica
5
125
DEAE
H
2
PO
4
-
10,000
ND
> 0.3
11.2
S
DEAE-3SW
Silica
10
250
DEAE
Cl -
30,000
ND
> 0.3
11.2
S
Sugar AXI
PS-DVB
8
60
Trimethyl-amino
HBO
3
-
ND
> 1.2
12.5
S
Sugar AXG
PS-DVB
10
60
Trimethyl-amino
HBO
3
-
ND
> 1.2
12.5
S
SAX
PS-DVB
5
60
Trimethyl-amino
Cl -
ND
> 1.0
12.5
S
Properties of TSKgel Ion exchange columns