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Traditional RP columns TSKgel ODS-80T
S
/ ODS-80T
M
/ OCTYL-80T
S
/ CN-80T
S
HIGHLIGHTS
ODS-80 is prepared from spherical silica with 80 Å pores
Monomeric-bonded phase chemistry for optimal lot-to-lot
reproducibility
High (80T
M
) or complete (80T
S
) endcapping shields the silica surface
from participating in solute retention through ionic interaction
Particles contain 80 Å pores for fast mass transfer of solutes in the
100 to 6,000 Da MW range
Available in particle sizes of 5 µm, 10 µm, and 20 µm
Large surface area and high sample capacity
APPLICATIONS
TSKgel ODS-80T
M
Hydrophobic and hydrophilic peptides, synthetic peptides, purity
check, peptide mapping
General purpose column for low MW pharmaceuticals, basic
compounds, nucleosides, nucleotides, purines and pyrimidines
TSKgel ODS-80T
S
Complete endcapping makes the TSKgel ODS-80TS a good choice
for strongly basic compounds and for applications that require
operation at pH 7.5
TSKgel Octyl-80T
S
Faster kinetics than ODS, but lower hydrophobic selectivity
Lower hydrophobic selectivity of Octyl versus ODS
TSKgel CN-80T
S
Alternative toODS andOctyl columns for analysis of polar compounds
Solvent strength should be reduced to obtain similar retention to
Octyl and ODS columns when separating non-polar compound
s
Traditional RP columns TSKgel ODS-120A - TSKgel ODS-120T
HIGHLIGHTS
TSKgel ODS-120 contains polymeric-bonded octadecyl groups on
120Å pore size silica
TSKgel ODS-120A is not endcapped; TSKgel ODS-120T is endcapped
with trimethylsilyl groups
TSKgel 120T columns are available in 2 mm ID format
Available in 5 µm and 10 µm particle sizes in analytical and semi-
preparative columns respectively. Larger particle sizes are available
in preparative columns
Hardware: stainless steel columns for analytical, semi-preparative,
and preparative separations
APPLICATIONS
TSKgel ODS-120A
Polymeric bonded ODS exhibits improved peak shape for the
separation of complex geometric isomers, such as polynuclear
aromatic hydrocarbons (PAH)
TSKgel ODS-120A and 120T provide a similar separation at low pH
for a mixture of catecholamines, while at pH 6 the basic solutes
interact with negatively charged silanol groups on 120A, but not on
120T
TSKgel ODS-120T
Endcapped ODS-120T is an alternative to ODS-80T
M
for peptide and
protein separations