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14
RPC
HIGHLIGHTS
The silica particles used in Super series columns are monodisperse
spherical 2.3 µm beads with 110 Å pores
TSKgel Super-ODS, Super-Octyl and Super-Phenyl packings are
bonded with, respectively, C18, C8 and phenyl functional groups.
The bonded phases have a polymeric structure. An exhaustive
endcapping reaction minimizes the presence of residual silanol
groups
2 µm particles provide superior resolution and speed, as well as
improved sensitivity
Pressure drop is not excessive due to the monodisperse particle
size distribution
APPLICATIONS
TSKgel
Super-ODS, Super-Octyl, Super-Phenyl
Recommended for small molecular weight compounds (<10,000 Da) such
as peptides, amino acids, tryptic digests, nucleotides, pharmaceutical
molecules, and food and beverage samples.
Optimizing Results with Fast RP Columns
Super series columns can be used on a regular HPLC system if the
dead volume is minimized, although optimal results are obtained with an
UHPLC system.
The following recommendations are for 4.6 mm ID columns. Use propor-
tionately lower values for 2 mm ID columns.
1.
A guard filter is highly recommended to reduce particulate
contamination from the sample or system components.
2. Keep sample volume less than 10 µL.
3. To ensure minimal extra-column volume, keep tubing as short as
possible (extra-column volume less than 5 µL between column and
detector).
4. Conventional 0.1 mm ID connecting tubing may be used
(0.005 ).
5. The smallest detector time constant should be selected
(if possible, less than 50 ms).
6. The detector flow cell should be 2 µL or less for best results. A
standard HPLC flow cell (10 µL) can be used as an alternative,
however, it is recommended that the heating coil is removed.
Fast RP Columns TSKgel SUPER-ODS / SUPER-OCTYL / SUPER PHENYL
Part #
Description
ID Length Particle
Number
Flow rate (mL/min)
Maximum
(mm)
(cm)
size (µm)
theoretical
Range
Max.
pressure
plates
drop (MPa)
TSKgel Stainless Steel Columns
20015 Super-ODS, 110 Å
1.0
5.0
2.3
≥
15,000 0.03 - 0.05
0.06
15.0
19541 Super-ODS, 110 Å
2.0
5.0
2.3
≥
6,000 0.15 - 0.2
0.25
25.0
19542 Super-ODS, 110 Å
2.0
10.0
2.3
≥
12,000 0.15 - 0.2
0.25
25.0
18154 Super-ODS, 110 Å
4.6
5.0
2.3
≥
8,000 1.0 - 2.5
4.0
30.0
18197 Super-ODS, 110 Å
4.6
10.0
2.3
≥
16,000 1.0 - 2.5
4.0
30.0
20013 Super-Octyl, 110 Å
2.0
5.0
2.3
≥
15,000 0.15 - 0.20
0.25
15.0
20014 Super-Octyl, 110 Å
2.0
10.0
2.3
≥
1,500 0.15 - 0.20
0.25
30.0
18275 Super-Octyl, 110 Å
4.6
5.0
2.3
≥
8,000 1.0 - 2.5
4.0
30.0
18276 Super-Octyl, 110 Å
4.6
10.0
2.3
≥
16,000 1.0 - 2.5
4.0
30.0
20017 Super-Phenyl, 110 Å
2.0
5.0
2.3
≥
3,000 0.15 - 0.20
0.25
8.0
20018 Super-Phenyl, 110 Å
2.0
10.0
2.3
≥
6,000 0.15 - 0.20
0.25
15.0
18277 Super-Phenyl, 110 Å
4.6
5.0
2.3
≥
8,000 1.0 - 2.5
4.0
30.0
18278 Super-Phenyl, 110 Å
4.6
10.0
2.3
≥
16,000 1.0 - 2.5
4.0
30.0
Guard column products
19672 Guard cartridge, pk 3*
2.0
1.0
2.3
For 2 mm ID Super-ODS columns
19308 Cartridge holder
For P/N 19672
18207 Guard filter, pk 3*
4.0
0.4
For 4.6 mm ID columns (Super-ODS, -Octyl, -Phenyl)
18206 Guard filter holder
For P/N 18207
ORDERING INFORMATION
*needs cartridge holder