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APPENDIX
APPENDIX
APPENDIX C
United States Pharmacopeia (USP) specifications and corresponding
Tosoh Bioscience columns
L1 - Octadecyl silane chemically bonded to porous silica or ceramic
micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod.
Recommendations: TSKgel ODS-100V, ODS-100Z, ODS-100S,
Super-ODS, ODS-80TM, ODS-80TS, ODS-120A, ODS-120T
See: Reversed Phase section
L7 - Octylsilane chemically bonded to totally porous silica particles, 1.5
to 10 µm in diameter.
Recommendations: TSKgel Super-Octyl, Octyl-80TS
See: Reversed Phase section
L-9 - Irregular or spherical, totally porous silica gel having a
chemically bonded, strongly acidic cation-exchange coating, 3 to
10 µm in diameter.
Recommendations: TSKgel SP-2SW
See: Ion Exchange section
L10 - Nitrile groups chemically bonded to porous silica particles, 3 to
10 µm in diameter.
Recommendations: TSKgel CN-80TS
See: Reversed Phase section
L11 - Phenyl groups chemically bonded to porous silica particles, 1.5
to 10 µm in diameter.
Recommendations: TSKgel Super-Phenyl
See: Reversed Phase section
L13 - Trimethylsilane chemically bonded to porous silica particles, 3 to
10 µm in diameter.
Recommendations: TSKgel TMS-250
See: Reversed Phase section
L20 - Dihydroxypropane groups chemically bonded to porous silica
particles, 5 to 10 µm in diameter.
Recommendations: TSKgel QC-PAK 200 and 300, SW
xl
series, SW
series
See: Size Exclusion section
L21 - A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm
in diameter
Recommendations: TSKgel H
xl
and H
hr
series
See: Size Exclusion section
L22 - A cation-exchange resin made of porous polystyrene gel with
sulfonic acid groups, about 10 µm in size
Recommendations: TSKgel SCX
See: Ion Exchange section
L23 - An anion-exchange resin made of porous polymethacrylate or
polymethacrylate gel with quartenary ammonium groups, about 10
µm in size.
Recommendations: TSKgel SuperQ-5PW, BioAssist Q, IC-Anion PW
See: Ion Exchange section
L24- A semi-rigid hydrophilic gel consisting of vinyl polymers with
numerous hydroxyl groups in the matrix surface, 32 to 63 µm in
diameter.
Recommendations: TOYOPEARL HW-type
See: Size Exclusion in the Bulk Resin section
L25- Packing having the capacity to separate compounds with
a molecular weight range from 100-5000 (as determined by
polyethylene oxide), applied to neutral, anionic, and cationic
water-soluble polymers.
Recommendations: TSKgel G2500PW, G2500PW
xl
, Alpha-2500,
SuperAW2500
See: Size Exclusion section
L33- Packing having the capacity to separate dextrans by molecular
size over a range of 4,000 to 500,000 daltons. It is spherical, silica-
based, and processed to provide pH stability.
Recommendations: TSKgel SuperSW, SW
xl
, QC-PAK, and SW
series
See: Size Exclusion section
L37- Packing having the capacity to separate proteins by
molecular size over a range of 2,000 to 40,000 daltons. It is a
polymethacrylate gel.
Recommendations: TSKgel G3000PW
xl
, G3000PW,
See: Size Exclusion section
L38- A methacrylate-based size-exclusion packing for water-soluble
samples
Recommendations: TSKgel PW
xl
, PW, Alpha, and SuperAW series
See: Size Exclusion section
L39- A hydrophilic polyhydroxymethacrylate gel of totally porous
spherical resin.
Recommendations: TSKgel PW, PW
xl
, Alpha, and SuperAW series
See: Size Exclusion section
L52- A strong cation exchange resin made of porous silica with
sulfopropyl groups, 5 to 10 µm in diameter.
Recommendations: SP-2SW
See: Ion Exchange section
L58- Strong cation-exchange resin consisting of sulfonated cross-
linked styrene-divinylbenzene copolymer in the sodium form, about
7 to 11 µm diameter.
Recommendations: TSKgel SCX (Na
+
)
See: Ion Exchange section