1. What is the support material for Tosoh Bioscience affinity resins?
TSKgel affinity chromatography columns are based upon the G5000PW methacrylate porous resin. This “5PW” media is hydrophilic, with 100 nanometer (nm) pores and an estimated protein size exclusion limit of 1000 kilodaltons.
2. What are the ligand concentrations, adsorption capacities and test analytes for the various affinity column supplied by Tosoh Bioscience?
| Characteristics of TSKgel AFC Columns |
|
| Column Packing |
Ligand Type |
Ligand Concentration |
Adsorption Capacity |
Sample |
| Boronate-5PW |
m-aminophenyl boronate |
not available |
40 µmol/mL - resin |
sorbitol |
| Chelate-5PW |
iminodiacetic acid |
20 µmol/mL - resin |
not available |
not available |
| Tresyl-5PW |
tresyl |
ca. 20 µmol/mL - resin |
> 60 mg/g dry resin (coupling capacity) |
soybean trypsin inhibitor |
3. Can I get a TSKgel Affinity LC column with non-metal wetted parts?
Yes. We supply glass columns in two formats: 5 mm ID x 5 cm L and 8 mm ID x 7.5 cm L with 10 µm pore size Pyrex frits.
The shipping solvents for these columns are as follows:
| Boronate-5PW:
|
distilled water |
| Chelate-5PW: |
acetate buffer |
| Tresyl-5PW: |
acetone |
4. How do I load and desorb protein from a TSKgel affinity column?
Specific instructions for retaining your protein as well as desorption procedures are included with each affinity column. Keep in the mind that the analyte requires some structural feature that coincides with the retention mechanism of the affinity resin.