TSKgel Octadecyl-2PW
TSKgel Octadecyl-2PW is based on a highly cross-linked polymethacrylate base matrix bonded with octadecyl groups. The polymeric matrix provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base.
The 12.5 nm (125 Å) pore size of TSKgel Octadecyl-2PW columns makes them ideally suited for peptides and small proteins. They demonstrate faster analysis than other competitive reversed phase polymeric columns.
TSKgel Octadecyl-2PW structure
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Separation of eight peptides
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Column: TSKgel Octadecyl-2PW, 5 µm, 4.6 mm ID × 15 cm
Mobile phase: 30 min linear gradient from 0.1% TFA/CH3CN
from 90/10 to 30/70
Flow rate: 1.0 mL/min
Detection: UV @ 215 nm
Temperature: ambient
Samples: 1. met-enkephalin, 2. bradykinin
3. leu-enkephalin, 4. neurotensin
5. bombesin, 6. angiotensin I
7. somatostatin, 8. insulin (bovine) |