TSKgel SuperQ-5PW
TSKgel SuperQ-5PW columns are packed with porous hydrophilic polymer beads which are modified with a strong anion exchange group. The resulting product exhibits very high protein capacities. TSKgel SuperQ-5PW columns are used for the separation and analysis of proteins, oligonucleotides and other biologically active molecules.
Stability of TSKgel SuperQ-5PW columns |
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Column: TSKgel SuperQ-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: A: 50 mmol/L Tris-HCI, pH 8.6
B: 0.5 mmol/L sodium cloride in 50 mmol/L Tris-HCI,
pH 8.6
Gradient: A-B (60 min)
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 100 µL
Sample load: each of 1 mg
Samples: 1. ovalbumin, 2. trypsin inhibitor
Note: A: before CIP, B: after 15 times (15 days) |
Analysis of 16-mer oligonucleotide |
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Column: TSKgel SuperQ-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: A: 10 mmol/L NaOH
B: 10 mmol/L NaOH with 1 mol/L NaCl
Gradient: 0 min (0%B) 40 min (50%B)
41 min (100%B) 46 min (100%B)
Flow Rate: 1 mL/min
Detection: UV @ 254 nm
Sample: 16-mer morpholine oligonucleotide,
AAG AAG AAG AGG GGA G
Sample load: 0.5 O.D. (optical density) |
Comparison of various anion exchange columns under large sample load |
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Columns: A: TSKgel SuperQ-5PW, 10 µm, 5 mm ID × 5 cm
B: Company A, Q type, 5 mm ID × 5 cm
C: Company A, perfusion Q type, 6.4 mm ID × 3 cm
All column volumes were 1.0 mL
Mobile phase: A: 50 mmol/L Tris-HCl buffer, pH 8.3
B: A + 0.5 mol/L NaCl
A to B linear gradient (60 min)
Flow rate: 0.8 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 2 mL
Samples: ovalbumin, 20 mg
trypsin inhibitor, 20 mg |