TSKgel SP-STAT
TSKgel SP-STAT columns are packed with 7 and 10 µm nonporous resin particles of which the surface consists of long hydrophilic chains of open access network of multi-layered sulfopropyl cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure.
Applications for the TSKgel SP-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.
Fast separation of protein standards |
 |
Columns: A: TSKgel SP-STAT, 10 µm, 3.0 mm ID × 3.5 cm
B: ProSwift® SCX-1S Monolith, 4.6 mm ID × 5 cm
Mobile phase: A: 20 mmol/L sodium acetate, pH 5.0
B: 1.0 mol/L NaCl in mobile phase A, pH 5.0 for column A
1.5 mol/L NaCl in mobile phase A, pH 5.0 for column B
Gradient: 0 min (0%B) 1 min (100%B)
Flow rate: A: 2.0 mL/min, B: 4.73 mL/min
Detection: UV @ 280 nm
Samples: 1. α-chymotrypsinogen A
2. cytochrome C, 3. lysozyme |
Monitoring of reaction products |
 |
Column: TSKgel SP-STAT, 10 µm, 3 mm ID × 3.5 cm
Mobile phase: A: 20 mmol/L sodium acetate buffer, pH 5.0
B: 1.0 mol/L NaCl in mobile phase A, pH 5.0
Gradient: 0 min (0%B) 2 min (100%B)
Flow rate: 2.0 mL/min
Detection: UV @ 280 nm
Sample: pegylated β-lactoglobulin |