TSKgel DNA-NPR
The TSKgel DNA-NPR column is packed with 2.5 µm, nonporous, hydrophilic polymer beads which are surface modified with a weak anion exchange group. Because this column contains non-porous particles, binding capacity is low compared to porous columns with the same ligand functionality, whereas protein recovery and efficiency is generally very high.
The TSKgel DNA-NPR column dimensions are optimized for the high efficiency separation of DNA fragments, PCR products, or plasmids.
Plasmid Analysis |
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Column: TSKgel DNA-NPR, 2.5 µm, 4. 6 mm ID × 7.5 cm
Mobile phase: A. 20 mmol/L Tris, pH 9.0
B. 20 mmol/L Tris, pH 9.0 with 1 mol/L NaCl
Gradient: linear gradient from 50% to 65%B in 10 column volumes
Flow rate: 1 mL/min
Detection: UV @ 260 nm
Sample: PUC19 plasmid |
Plasmid Analysis |
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Column: TSKgel DNA-NPR, 2.5 µm, 4.6 mm ID × 7.5 cm
Mobile phase: A: 10 mmol/L sodium bromide, 20 mmol/L NaOH,
pH 12, 1% diethylamine
B: 1 mol/L sodium bromide, 20 mmol/L NaOH,
pH 12, 1% diethylamine
Gradient: 3.5 min (20%B) 12 min (20%B) 45 min (55%B)
Flow rate: 1.0 mL/min
Temperatures: 60 °C (column), 4 °C (sample chamber)
Sample: crude deprotected 13-mer oligonucleotide |