TSKgel DEAE-5PW
TSKgel DEAE-5PW columns are packed with porous, hydrophilic polymer beads which are surface modified with a weak anion exchange group. These columns are used for the separation and analysis of proteins, along with nucleotides, nucleosides, and other biomolecules.
TSKgel DEAE-5PW columns are available in internal diameters varying from 2 mm to 21.5 mm and in column housings of either glass or stainless steel.
Analysis of high MM RNA
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Column: TSKgel DEAE-5PW, 10 µm, 6 mm ID × 15 cm (custom)
Mobile phase: 300 min linear gradient from 0.3 mol/L to
1.0 mol/L NaCl in 0.1 mol/L Tris-HCl, pH 7.6
Flow rate: 1.0 mL/min
Detection: UV @ 260 nm
Sample: total E. coli RNA |
Detection of HIV-1 PCR-Amplified 130 bp target
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Column: TSKgel DEAE-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: A: 25 mmol/L Tris-HCl, 1 mmol/L EDTA, pH 8.0
B: 25 mmol/L Tris-HCl, 1 mmol/L EDTA, pH 8.0,
with 1 mol/L NaCl
Gradient: 25-60%B in 30 minutes
Flow rate: 1 mL/min
Detection: UV @ 260 nm
Temperature: 25 °C
Sample: pBR322 plasmid
Sample load: 2.5 mg in 1 mL |
Separation of monoclonal antibody
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Column: TSKgel DEAE-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: A: 20 mmol/L Tris-HCI, pH 8.5
B: A + 0.5 mol/L NaCl
A to B linear gradient (60 min)
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 ºC
Sample: anti-human albumin (lgG1),
diluted solution of mouse ascites (168 µg in 40 µL) |