TSKgel BioAssist Q
TSKgel BioAssist Q columns are packed with polymethacrylate particles containing very large pores (~400 nm) that are functionalized with polyamine groups to form a network structure. The very large pores allow large biomolecules to freely enter and exit the pores resulting in higher binding capacity and better recovery of activity than other competitive materials. The capacity of the TSKgel BioAssist Q columns is high over a wide molecular weight range (up to 1.0 × 10^6 Da).
The standard TSKgel BioAssist Q column is 4.6 mm ID x 5 cm, while a 10 mm ID x 10 cm semi-preparative column is available for scale-up. Both columns are made of PEEK to reduce protein adsorption.
Analysis of mouse ascites fluid
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Columns: A: TSKgel BioAssist Q, 10 µm, 4.6 mm ID × 5 cm
B: Commercial Q-type product A, 5.0 mm ID × 5 cm
Mobile phase: 15 min linear gradient of NaCl from 0 to 1.0 mol/L
in 20 mmol/L Tris-HCl buffer, pH 8.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 ºC
Injection vol.: 5 µL
Sample: mouse ascites fluid (3-fold dilution with initial eluent)
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Performance enhancement
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Columns: A: TSKgel BioAssist Q, 10 µm, 4.6 mm × 5 cm
B: Competitor Q, 5.0 mm ID × 5 cm
Mobile phase: 30 min linear gradient from 0 to 1 mol/L NaCl
in 20 mmol/L sodium phosphate, pH 8.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: 1. conalbumin
i. ovalbumin impurity
2. ovalbumin
3. trypsin inhibitor
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Dynamic binding capacity as function of protein molar mass
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Columns: TSKgel BioAssist Q, 10 µm, 4.6 mm ID × 1 cm
Conventional Q-type product A, 5.0 mm ID × 1 cm
TSKgel SuperQ-5PW, 4.6 mm ID × 1 cm
Mobile phase: 20 mmol/L Tris-HCI buffer, pH 8.0
Flow rate: 0.38 mL/min
Detection: UV @ 280 nm
Temperature: 25 ºC
Samples: 1. trypsin inhibitor, 10 g/L
2. human serum albumin, 10 g/L
3. lgG1, 2.3 g/L
4. thyroglobulin, 5 g/L |