TSKgel Ether-5PW
TSKgel Ether-5PW is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with polyether groups. The TSKgel Ether-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.
TSKgel Ether-5PW is less hydrophobic than Phenyl-5PW and the best choice for the separation of very hydrophobic proteins or monoclonal antibodies.
Purification of anti-tumor antibiotic
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Column: TSKgel Ether-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: linear gradient from 1.5 mol/L to 0 mol/L (NH4)2SO4
in 0.1 mol/L phosphate buffer, pH 7.0
Flow rate: 0.8 mL/min
Detection: UV @ 220 nm
Injection vol.: 20 µL
Sample: C-1027 (red line), C-1027-AG (blue line) concentration: 1 g/L |