TSKgel Boronate-5PW
TSKgel Boronate-5PW is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with m-aminophenyl boronate. This ligand is capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, glycosolyated proteins, and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. Applications include nucleic acids, catecholamines, nucleosides and nucleotides.
TSKgel Boronate-5PW structure
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Isocratic separation of nucleosides
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Column: TSKgel Boronate-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: 0.1 mol/L phosphate buffer, pH 8.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: 1. cytidine, 2. uridine
3. guanosine, 4. adenosine |