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AFC
AFC
89
ORDERING INFORMATION
Part # Description
ID
Length
Particle
Number
Flow rate (mL/min)
Maximum
(mm)
(cm)
size (µm)
theoretical
Range
Max.
pressure drop
plates
(MPa)
Glass columns
14449 Boronate-5PW Glass, 1000 Å
5.0
5.0
10
≥
500
0.5 - 1.0
1.2
2.0
14440 Chelate-5PW Glass, 1000 Å
5.0
5.0
10
≥
500
0.5 - 0.8
1.0
2.0
TSKgel Stainless Steel Columns
13066 Boronate-5PW, 1000 Å
7.5
7.5
10
≥
1,300
0.5 - 1.0
1.2
1.0
08645 Chelate-5PW, 1000 Å
7.5
7.5
10
≥
1,300
0.5 - 1.0
1.2
1.0
14455 Tresyl-5PW, 1000 Å
6.0
4.0
10
0.2 - 0.5
1.0
1.0
14456 Tresyl-5PW, 1000 Å
7.5
7.5
10
0.5 - 1.0
1.2
1.0
TSKgel PEEK columns
20022
BioAssist Chelate, 1000 Å
7.8
5.0
10
≥
800
0.5 - 1.0
1.2
1.0
Guard column products
14451 Boronate-5PW Glass Guardgel Kit
10
For P/N 14450 and 14449
13125 Boronate-5PW Guardgel Kit
For P/N 13066
08647 Chelate-5PW Guardgel Kit
For P/N 08645
Bulk packing
16208 Tresyl-5PW, 2 g dry gel*
* 1 g is approximately 3.5 mL
Principal applications for TSKgel Tresyl-5PW include the selective
purification of antigens after coupling the appropriate antibody to the
solid support. The antibody coupling yield at pH >7.5 is more than 90 %,
with the maximum binding occurring at pH 7.5. Antigen adsorption to the
antibody ligand is most effective when the antibody concentration is
< 2-3 mg/mL of affinity resin. To increase binding capacity, more
antibody should be added to the coupling reaction.
However, higher concentrations of antibody can result in steric
hindrance, thus lowering the binding capacity of the column.
As a general rule, the time required for antibody attachment to
the TSKgel Tresyl-5PW column is directly proportional to the
antibody concentration. Small amounts of antibody require about
2 hours to complete the cross-linking reaction, whereas it may
take 6-7 hours to fully attach an antibody at the concentration of
10 mg/mL-resin.
Examples of the wide range of applications using TSKgel Tresyl-5PW
include the binding of such ligands as concanavalin A (a lipoprotein
lectin that binds to glycoproteins), numerous antibodies and enzymes.
The chromatogram in
Figure 4
shows the purification of peroxidase
by the concanvalin A ligand coupled to the TSKgel Tresyl-5PW affinity
support resin.