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tosoh bioscience
chromatography
catalog
Introduction
Tosoh Bioscience is a major supplier of liquid chromatography pro-
ducts worldwide, particularly to the pharmaceutical and biotechnology
industries. The company distributes and supports products manufac-
tured by our parent company, Tosoh Corporation, which has offices and
manufacturing facilities in Japan. Located in Stuttgart, Germany, Tosoh
Bioscience GmbH provides sales and service to customers in Europe,
the Middle East, South Asia and Africa.
This Chromatography Products Catalog describes analytical and semi-
preparative TSKgel
®
prepacked columns for each of the major chro-
matography modes. It also gives a short overview of TOYOPEARL
®
and
TSKgel bulk resins for laboratory scale purifications, as well as Toyo-
Screen process development columns.
TSKgel and TOYOPEARL products are used for the analysis, isolation
and purification of proteins, peptides, DNA, oligonucleotides, antibiotics
and other small molecular weight compounds. Our products are known
for their ability to withstand high pressure, their robust chemical stabil-
ity, and their superior performance for the recovery, concentration and
purification of biomolecules.
What’s new
A new TSKgel PW column line incorporating the company’s proprietary
multi-pore particle technology is extending the TSKgel SEC column
portfolio. TSKgel SuperMultiporePW semi-micro SEC columns provide
near linear calibration curves and are ideally suited to analyze the size
distribution of water soluble polymers, such as polyvinylpyrrolidones or
dextrans. The series comprises of three column types covering different
molecular weight ranges.
A new, robust amino phase, TSKgel NH2-100, is expanding the selectivity
range of TSKgel HILIC solutions. This HILIC phase is well suited for all
separations requiring amino functional groups. In contrast to many
other amino phases the new column offers expanded stability under
HILIC conditions. It is based on a 3 µm silica particle with 100 Å pores,
which is treated with a proprietary endcapping procedure. Amino
groups are introduced after endcapping and act as HILIC functional
groups without any peak splits. Due to a high ligand density and large
surface area TSKgel NH2-100V 3µm columns show high retention for
very polar compounds.
The new TOYOPEARL AF-rProtein A-650F affinity resin is well-suited
for high capacity capturing of Antibodies. It binds human and mouse
immunoglobulin G (IgG) and Fab fragments. The resin combines a high
hIgG dynamic binding capacity at short residence time and fast mass
transfer kinetics with an improved alkaline stability. It provides a typical
dynamic binding capacity for IgG of more than 30 mg/mL resin at two
minutes residence time.
TOYOPEARL Q-600C AR is a new anion exchange resin with an average
particle size of 100 µm. It is well-suited for high capacity capture of
biotherapeutics or plasma proteins. ‘AR’ stands for alkaline resistance
as the resin combines high dynamic binding capacity and fast mass
transfer kinetics with an improved alkaline stability.
The new TSKgel SP-3PW (30) cation exchange resin was designed as
a polishing resin with improved binding capacities for peptides and
small proteins. It is a strong cation exchange resin having a smaller
pore size than the corresponding TSKgel SP-5PW material and also a
different selectivity. A typical DBC of about 50 g/L for insulin makes this
resin attractive for all peptide purification tasks that involve a cation
exchange step.