Basic HTML Version
96
BULK
INTRODUCTION TO BULK RESINS FOR LABORATORY PURIFICATION
Tosoh Bioscience offers TOYOPEARL and TSKgel resins (media) in bulk
quantities for laboratory-scale applications.
Although the resins can be applied to the purification of small as well as
large MW compounds, TOYOPEARL and TSKgel resins are most useful
for the separation of peptides, proteins, and oligonucleotides.
The focus of this section is on the use of bulk resins in laboratory
applications. Please request the Process Chromatography
catalog for information about the use of TOYOPEARL and
TSKgel for larger scale separations or visit our website at:
TOYOPEARL Bulk Resin
TOYOPEARL resins are hydrophilic, macroporous media for medium
pressure liquid chromatographic applications.
The polymethacrylate backbone structure of TOYOPEARL packings
assure excellent pressure/flow characteristics. TOYOPEARL is
mechanically stable up to 0,3 MPa, which simplifies column packing by
reducing the setup time and improving reproducibility from column to
column.
The media is stable over the range of pH 2-12 for normal operating
conditions and pH 1-13 for cleaning conditions. In most modes,
TOYOPEARL is available in three grades, S (superfine) for highest
performance, F (fine) and M (medium) for economical purification, and C
(coarse) and EC (extra coarse) for capture. Consult
Table I
for particle
sizes associated with the various chemistries and pore sizes.
FEATURES
BENEFITS
chemistries available in Size Exclusion, Ion Exchange,
Hydrophobic Interaction and Affinity chromatography
added flexibility during method development
methacrylate backbone has hydrophilic surface properties
less non-specific adsorption
high recovery of proteins, enzymes, glycoproteins
TSKgel and TOYOPEARL bulk resin product lines feature the
same ligand and backbone chemistries from 20 µm to 150 µm
particle sizes
simplified scale up from laboratory separation to process
SEC product line available in 5 pore sizes
suitable for fractionation of large and small biopolymers
IEC, HIC and AFC products are based on 1000 Å, 750 Å and 500 Å
pore size particles.
high capacity and efficient chromatography of small protein
and large biopolymers due to unrestricted access of available
surface area
chemical stability
cleanable resins in strong base or acid (pH 1-13)
compatible with all water soluble organic solvents
stable in chaotropic agents such as: guanidine hydrochloride,
sodium dodecyl sulfate and urea
thermal stability
autoclavable at 120°C
wide range of operating temperature (4-60°C)
mechanical stability
linear relationship between flow rate and pressure drop
column bed stability
constant bed volume over a wide range of salt concentrations