Selecting the right particle size:
A customer's feedstock, its viscosity, the amount of particulate, and the operational pressure limits of the chromatographic hardware used, determine the most appropriate particle size for a customer's capture step. The more viscous and particulate laden the feedstock, the larger the particle needed to produce a linear velocity suitable for the process throughput objectives.
Many commercial Toyopearl and TSK-GEL PW bulk resin products are offered in particle sizes from 100 µm to 20 µm and have the same methacrylic polymer backbone. The selectivity of feedstock components on these resins remains the same regardless of bead size.
Toyopearl resins with bead diameters of 35 µm and larger are made to withstand operating pressures up to 3 bar.
TSK-GEL PW resins with bead diameters of 30 µm and smaller are made to withstand operating pressures up to 20 bar.
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Tosoh Bioscience has commercial products with the following particle sizes for a typical capture step:
| Grade |
Particle size range
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| EC (Extra Coarse) |
100-300 µm
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| C (Coarse) |
50-150 µm
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| M (Medium) |
40-90 µm
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Approximate dynamic binding capacities for capture resins:
(please click on the mode of interest for more product information)
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