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Tosoh Bioscience GmbH: The Chemistry of Innovation
Process Media Products
Toyopearl Media
Process Media Products
Size Exclusion (SEC)
Ion Exchange (IEC)
Toyopearl CM-650
Toyopearl DEAE-650
Toyopearl GigaCap CM-650
Toyopearl GigaCap Family - Reduction of Elution Volume
Toyopearl GigaCap Q-650
Toyopearl GigaCap S-650
Toyopearl MegaCap II SP-550EC
Toyopearl Q-600 AR
Toyopearl QAE-550
Toyopearl SP-550
Toyopearl SP-650
Toyopearl SuperQ-650
TSKgel DEAE-5PW
TSKgel SP-3PW
TSKgel SP-5PW
TSKgel SuperQ-5PW
Hydrophobic Interaction (HIC)
Affinity (AFC)


Toyopearl SP-650 is a strong cation exchanger for biomolecule purification.  It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed the smaller particle TSK-GEL SP-5PW should be used. Selectivity remains the same.

 Structure:





 Product Attributes:
Pore size (mean): 1,000 Å

Particle size (mean): 100 µm (C-grade)
  65 µm (M-grade)
  35 µm (S-grade)

Pressure rating: 3 bar

Shipping buffer: 20% ethanol

pH stability: 2-13

Shelf life: 10 years

 Literature:
Process Media Catalog - Ion Exchange Chromatography

Product Specification Sheet -
Toyopearl SP-650


Toyopearl Instruction Manual


 Comparative information for CIEX resins
Influence of pore size on Lysozyme DBC for
Toyopearl cation exchangers @ 100 cm/hour



pKa's for Ion Exchange groups

pKa's for IEX resins


Ordering information:

To place an order please contact Customer Service.

P/N Description Resin Volume Pore size

Particle size

07994 Toyopearl SP-650C 250 mL 1,000 Å
100 µm

14700 Toyopearl SP-650C 1 L 1,000 Å
100 µm

07995 Toyopearl SP-650C 5 L 1,000 Å
100 µm

43202 Toyopearl SP-650M 100 mL 1,000 Å
65 µm

07997 Toyopearl SP-650M 250 mL 1,000 Å
65 µm

14699 Toyopearl SP-650M 1 L 1,000 Å
65 µm

07998 Toyopearl SP-650M 5 L 1,000 Å
65 µm

19822 Toyopearl SP-650S 25 mL 1,000 Å
35 µm

08437 Toyopearl SP-650S 250 mL 1,000 Å 

35 µm

14698 Toyopearl SP-650S 1 L 1,000 Å 35 µm

08438 Toyopearl SP-650S 5 L 1,000 Å  35 µm

21368 ToyoScreen SP-650M 6 x 1 mL 1,000 Å  65 µm

21369 ToyoScreen SP-650M 6 x 5 mL 1,000 Å  65 µm

21394 ToyoScreen IEC Cation Mix Pack,
(GigaCap CM-650M, GigaCap S-650M, CM-650M,
SP-650M, SP-550M)
5 x 1 mL 1,000 Å  35 µm

21395 ToyoScreen IEC Cation Mix Pack,
(GigaCap CM-650M, GigaCap S-650M, CM-650M,
SP-650M, SP-550M)
5 x 5 mL 1,000 Å  35 µm


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NEW! Toyopearl & TSKgel
Process Media Catalog


Toyopearl Orientation Sheet

Toyopearl Brochure (en francais)

Process Media Main Page

Tosoh Bioscience GmbH Zettachring 6, 70567 Stuttgart, Germany  Tel: +49-711-132570  Email: info.tbg@tosoh.com