Toyopearl GigaCap S-650 is a high capacity cation exchange resin* optimized specifically for the capture and purification of IgG. Toyopearl HW-65 is the polymeric base bead chemically modified to provide a high level of cationic binding sites for IgG. It exhibits typical dynamic binding capacities in the vicinity of 140 mg/mL for IgG. This new polymer product is stable at high linear velocities, has good pressure flow characteristics and will withstand back pressures up to 3 bar. The new higher capacity and back pressure stability of Toyopearl GigaCap S-650 creates opportunities for increased throughput in downstream cation exchange purification steps.
While Toyopearl GigaCap S-650 was specifically optimized for IgG, it also exhibits high binding capacities for smaller proteins. Typical dynamic binding capacities for lysozyme are in excess of 160 mg/mL.
Toyopearl GigaCap S-650M also has excellent base stability in 1.0 mol/L NaOH and shows robust clean in place properties with minimal loss of capacity after 50 x 1 hour cycles of caustic exposure also using 1.0 mol/L NaOH (data).
*specifically designed for packed bed use |