Toyopearl Phenyl-650

Size Exclusion (SEC)
Ion Exchange (IEC)
Hydrophobic Interaction (HIC)
	Toyopearl Butyl-600
	Toyopearl Butyl-650
	Toyopearl Ether-650
	Toyopearl Hexyl-650
	Toyopearl Phenyl-600
	Toyopearl Phenyl-650
	Toyopearl PPG-600
	Toyopearl SuperButyl-550
	TSKgel Ether-5PW
	TSKgel-Phenyl-5PW
Affinity (AFC)

Toyopearl Phenyl-650 is the middle hydrophobicity of the five HIC ligands offered by Tosoh Bioscience. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps. For high performance separations, the use of TSK-GEL Phenyl-5PW is suggested. Both products have the same selectivity.

Structure:

Toyopearl Phenyl-650 chemical structure

Hydrophobicity

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Pore size (mean):	1,000 Å
Particle size (mean):	100 µm (C-grade) 65 µm (M-grade) 35 µm (S-grade)
Pressure rating:	3 bar
Shipping buffer:	20% ethanol
pH stability:	1-13
Shelf life:	10 years

Literature:

Process Media Catalog - Hydrophobic Interaction Chromatography

Toyopearl HIC Brochure

Product Specification Sheet -
Toyopearl Phenyl-650

Toyopearl Instruction Manual

Comparative information for HIC resins

Typical Dynamic Binding Capacities for Lysozyme

Selectivities of Toyopearl HIC resins

Ordering information:
To place an order please contact Customer Service.

P/N	Description	Resin Volume	Pore size	Particle size
43152	Toyopearl Phenyl-650S	25 mL	1000 Å	35 µm
14477	Toyopearl Phenyl-650S	100 mL	1000 Å	35 µm
14784	Toyopearl Phenyl-650S	1 L	1000 Å	35 µm
14935	Toyopearl Phenyl-650S	5 L	1000 Å	35 µm
19818	Toyopearl Phenyl-650M	25 mL	1000 Å	65 µm
14478	Toyopearl Phenyl-650M	100 mL	1000 Å	65 µm
14783	Toyopearl Phenyl-650M	1 L	1000 Å	65 µm
14943	Toyopearl Phenyl-650M	5 L	1000 Å	65 µm
43126	Toyopearl Phenyl-650C	25 mL	1000 Å	100 µm
14479	Toyopearl Phenyl-650C	100 mL	1000 Å	100 µm
14785	Toyopearl Phenyl-650C	1 L	1000 Å	100 µm
14944	Toyopearl Phenyl-650C	5 L	1000 Å	100 µm
21374	ToyoScreen Phenyl-650M	6 x 1 mL	1000 Å	65 µm
21375	ToyoScreen Phenyl-650M	6 x 5 mL	1000 Å	65 µm
21398	ToyoScreen HIC (PPG-600M, Butyl-600M, Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)	6 x 1 mL
21399	ToyoScreen HIC (PPG-600M, Butyl-600M, Phenyl-650M, Butyl-650M, Phenyl-600M, Hexyl-650C)	6 x 5 mL

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