Toyopearl Ether-650

Size Exclusion (SEC)
Ion Exchange (IEC)
Hydrophobic Interaction (HIC)
	Toyopearl Butyl-600
	Toyopearl Butyl-650
	Toyopearl Ether-650
	Toyopearl Hexyl-650
	Toyopearl Phenyl-600
	Toyopearl Phenyl-650
	Toyopearl PPG-600
	Toyopearl SuperButyl-550
	TSKgel Ether-5PW
	TSKgel-Phenyl-5PW
Affinity (AFC)

Toyopearl Ether-650 has the highest hydrophilicity of the HIC ligands offered by Tosoh Bioscience. It is useful for very hydrophobic molecules such as membrane proteins. It is typically used in intermediate purification and polishing steps. For high performance separations, the use of TSK-GEL Ether-5PW is suggested. Both products have the same selectivity.

Structure:

Hydrophobicity

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Pore size (mean):	1.000 Å
Particle size (mean):	65 µm (M-grade) 35 µm (S-grade)
Pressure rating:	3 bar
Shipping buffer:	20% ethanol
pH stability:	1-13
Shelf life:	10 years

Literature:

Process Media Catalog - Hydrophobic Interaction Chromatography

Toyopearl HIC Brochure

Product Specification Sheet -
Toyopearl Ether-650

Toyopearl Instruction Manual

Comparative information for HIC resins

Typical Dynamic Binding Capacities for Lysozyme

Selectivities of Toyopearl HIC resins

Ordering information:
To place an order please contact Customer Service.

P/N	Description	Resin Volume	Pore size	Particle size
43151	Toyopearl Ether-650S	25 mL	1.000 Å	35 µm
16172	Toyopearl Ether-650S	100 mL	1.000 Å	35 µm
16174	Toyopearl Ether-650S	1 L	1.000 Å	35 µm
16176	Toyopearl Ether-650S	5 L	1.000 Å	35 µm
19805	Toyopearl Ether-650M	25 mL	1.000 Å	65 µm
16173	Toyopearl Ether-650M	100 mL	1.000 Å	65 µm
16175	Toyopearl Ether-650M	1 L	1.000 Å	65 µm
16177	Toyopearl Ether-650M	5 L	1.000 Å	65 µm
21372	ToyoScreen Ether-650M	6 x 1 mL	1.000 Å	65 µm
21373	ToyoScreen Ether-650M	6 x 5 mL	1.000 Å	65 µm

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