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Tosoh Bioscience GmbH: The Chemistry of Innovation
Process Media Products
Toyopearl Media
Process Media Products
Size Exclusion (SEC)
Ion Exchange (IEC)
Hydrophobic Interaction (HIC)
Toyopearl Butyl-600
Toyopearl Butyl-650
Toyopearl Ether-650
Toyopearl Hexyl-650
Toyopearl Phenyl-600
Toyopearl Phenyl-650
Toyopearl PPG-600
Toyopearl SuperButyl-550
TSK-GEL Ether-5PW
TSK-GEL-Phenyl-5PW
Affinity (AFC)


Toyopearl Butyl-600
has the second highest hydrophobicity of the HIC ligands offered by Tosoh Bioscience. Its pore size is between that of Toyopearl Butyl-650 and SuperButyl-550. It has been optimized for the purification of monoclonal antibodies and is typically used as an intermediate purification or polishing step.

 Structure:


Toyopearl Butyl-600 chemical structure


 Hydrophobicity



Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)


 Product Attributes:
Pore size (mean): 750 Å

Particle size (mean): 65 µm (M-grade)

Pressure rating: 3 bar

Shipping buffer: 20% ethanol

pH stability: 1-13

Shelf life: 10 years

 Literature:

Process Media Catalog - Hydrophobic Interaction Chromatography

Product Overview - Toyopearl Butyl-600M

Toyopearl HIC Brochure

Product Specification Sheet -
Toyopearl Butyl-600


Toyopearl Instruction Manual


 Comparative information for HIC resins
Typical Dynamic Binding Capacities for humanized mAb

Dynamic binding capacities for mAb

Selectivities of Toyopearl HIC resins



Ordering information:
To place an order please contact Customer Service.

P/N Description Resin Volume Pore size

Particle size

21448 Toyopearl Butyl-600M 25 mL 750 Å
65 µm

21449 Toyopearl Butyl-600M 100 mL 750 Å
65 µm

21450 Toyopearl Butyl-600M 1 L 750 Å
65 µm

21451 Toyopearl Butyl-600M 5 L 750 Å
65 µm

21494 ToyoScreen Butyl-600M 1 mL x 6 (each)  750 Å
65 µm

21495 ToyoScreen Butyl-600M 5 mL x 6 (each) 750 Å
65 µm


Printer Friendly Version

Toyopearl Orientation Sheet

Process Media Main Page

Tosoh Bioscience GmbH Zettachring 6, 70567 Stuttgart, Germany  Tel: +49-711-132570  Email: info.sep.eu@tosoh.com