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Toyopearl AF-rProtein A-650F is a high capacity affinity resin designed for efficient capturing of monoclonal antibodies at production scale. The recombinant ligand is derived from one of the IgG binding domains of Protein A by optimizing amino acid sequence. It is expressed in E. coli, free of animal derived product. Multipoint attachment of this ligand to the rigid Toyopearl matrix reduces ligand leaching and enhances mechanical, thermal and alkali stability.
Typical static IgG binding capacity is > 45g/L and typical dynamic IgG binding capacity is > 30 g/L (at 2 minutes residence time, 1 g/L loading concentration ). Cleaning-in-place (CIP) is possible with 0.1 M and 0.5 M NaOH. After more than 100 CIP cycles with 0.1 M NaOH at 16 min contact time typically more than 90 % of initial dynamic binding capacity is retained. |
 Structure: |
HW65-rProtein A
Ligand: recombinant Protein a variant (E. coli)
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Product Attributes:
| Pore size (mean): |
1,000 Å
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| Particle size (mean): |
45 µm (F-grade)
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| Pressure rating: |
3 bar
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| Shipping buffer: |
20% ethanol
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| SBC: |
> 45 g/L
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| DBC: |
> 30 g/L
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| Toyopearl AF-rProtein A-650F provides |
 Efficient MAB capturing with high purity from high titer feedstocks
High IgG capacity over a wide range of velocities
High alkaline stability for effective cleaning and sanitization
Rigid polymethacrylate base matrix with ideal pressure flow characteristics
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| Binding capacity & Alkaline stability |
Binding capacity at 10 mg/mL
Dynamic IgG binding capacity at various residence times Breakthrough curves for IgG loading at a concentration of 10 mg/ml: typical DBC at 10% breakthrough for Toyopearl AF-rProtein A-605F (red): 33 mg/mL@ 60 cm/hr (5 min residence time) - 24 mg/mL @ 200 cm/hr (1.5 min residence time)
Alkaline stability 0.1/0.5 M NaOH
Chemical Stability of Toyopearl AF-rProtein A-650F IgG binding capacity is not significantly impaired by more than 100 CIP cycles with 0.1 M NaOH (red, 16 min contact time/cycle) and 0.5 M NaOH (grey, 17 min contact time/cycle). Dynamic binding capacity of hIgG determined at 10% breakthrough.
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Ordering information:
To place an order please contact Customer Service.
| P/N |
Description |
Resin volume |
Pore size
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Particle size
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| 22803 |
Toyopearl AF-rProtein-650F |
10 mL |
1,000 Å
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45 µm
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| 22804 |
Toyopearl AF-rProtein-650F |
25 mL |
1,000 Å
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45 µm |
| 22805 |
Toyopearl AF-rProtein-650F |
100 mL |
1,000 Å |
45 µm
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| 22806 |
Toyopearl AF-rProtein-650F |
1 L |
1,000 Å |
45 µm
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| 22807 |
Toyopearl AF-rProtein-650F |
5 L |
1,000 Å |
45 µm
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| 22808 |
Toyopearl AF-rProtein-650F |
50 L |
1,000 Å |
45 µm
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| 22809 |
ToyoScreen AF-rProtein-650F |
1 mL x 5 |
1,000 Å |
45 µm
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| 22810 |
ToyoScreen AF-rProtein-650F |
5 mL x 1 |
1,000 Å |
45 µm
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| 22811 |
ToyoScreen AF-rProtein-650F |
5 mL x 5 |
1,000 Å |
45 µm
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| 22815 |
ProteinA-R28 ELISA Kit
(2 MTP, Standards, Reagents)
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Kit |
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| 22836 |
ProteinA-R28 Standard,
10 mg/L (0.5 mL)
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Kit |
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