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Tosoh Bioscience GmbH: The Chemistry of Innovation
Process Media Products
Toyopearl Media
Process Media Products
Size Exclusion (SEC)
Ion Exchange (IEC)
Hydrophobic Interaction (HIC)
Affinity (AFC)
Toyopearl AF-Amino-650
Toyopearl AF-Blue HC-650
Toyopearl AF-Carboxy-650
Toyopearl AF-Chelate-650
Toyopearl AF-Epoxy-650
Toyopearl AF-Formyl-650
Toyopearl AF-Heparin HC-650
Toyopearl AF-Red-650
Toyopearl AF-Tresyl-650


Toyopearl AF-Epoxy-650 is an activated resin provided in dry form for the immobilization of protein ligands for affinity chromatography. It is used when high densities of low molecular weight molecules need to be attached. It is also useful when a conversion to other special functional groups is required prior to ligand immobilization. For instance, its hydrazide form is very useful for carbohydrates or glycoprotein ligands.

 Structure:





 Product Attributes:
Pore size (mean): 1,000 Å

Particle size (mean): 65 µm (M-grade)

Pressure rating: 3 bar

Shipping condition: dry

Storage temperature: ambient

 Literature:

Process Media Catalog - Affinity Chromatography

Product Specification Sheet -
Toyopearl AF-Epoxy-650M


Toyopearl Instruction Manual


 Coupling procedure





Ordering information:


To place an order please contact Customer Service.

P/N Description Resin Volume Ligand density Adsorption capacity

43402 Toyopearl AF-Epoxy-650M 5 g* 600 - 1000 µeq/g >60 mg/g**

08000 Toyopearl AF-Epoxy-650M 10 g* 600 - 1000 µeq/g

>60 mg/g**

08038 Toyopearl AF-Epoxy-650M 100 g* 600 - 1000 µeq/g

>60 mg/g**

18315 Toyopearl AF-Epoxy-650M 1,000 g* 600 - 1000 µeq/g

>60 mg/g**


*1 g yields approximately 3.5 mL
**Measured as amount of test protein coupled
per gram of dry gel.

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Toyopearl Orientation Sheet

Process Media Main Page

Tosoh Bioscience GmbH Zettachring 6, 70567 Stuttgart, Germany  Tel: +49-711-132570  Email: info.sep.eu@tosoh.com