TSKgel PWxl columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for TSKgel PWxl columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel PW page for typical samples and suitable eluents. Figure 1 shows the calibration curves for all TSKgel PWxl-type columns. Figure 2 shows the benefits of TSKgel PWxl columns over PW columns, namely the improved resolution due to the smaller particle sizes used in PWxl columns.

Figure 1: Polyethylene glycol and oxide calibration curves on TSKgel PWxl columns	Figure 2: Faster analysis and higher resolution with TSKgel PWxl columns

Although proteins can be analyzed on TSKgel PWxl columns, pore volumes are much smaller than on SW-type columns. Figure 3 demonstrates the limited separation power of TSKgel PWxl for protein samples. Specialty Columns TSKgel G-DNA-PW is available for the analysis of DNA fragments. Similarly, TSKgel G-Oligo-PW is the preferred column for the analysis of oligosaccharides. The mixed-mode column, TSKgel GMPWxl, has an extended linear calibration range, suitable for samples with a broad MW distribution and unknowns.

Figure 3: Protein Calibration curves for TSKgel PWxl