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Tosoh Bioscience GmbH: The Chemistry of Innovation
Gel Filtration Chromatography
TSK-GEL Columns
TSKgel PW
TSKgel PWxl
TSKgel PWxl-CP
TSKgel SuperMultiporePW
TSKgel SuperOligoPW


TSKgel SuperMultiporePW columns are packed with spherical, mono-disperse, polymethacrylate particles incorporating the proprietary multi-pore particle technology. Each particle each contains a wide range of pore sizes in order to provide near linear calibration curves. They belong to the semi-micro type of SEC columns (6 mm ID, 15 cm length) providing high theoretical plate numbers at half of the length of a conventional SEC column. The TSKgel SuperMultiporePW series comprises of three column types covering different molecular weight ranges (PW-N; PW-M, PW-H).
The main application area for TSKgel SuperMultiporePW columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. Figure 1 shows the near linear calibration curves for the three SuperMultiporePW-type columns. Figure 2 shows the benefits of TSKgel SuperMultiporePW columns over a combination of different conventional SEC columns, namely the improved resolution and the smooth peak shapes without any inflection points.

Product Attributes:

  TSKgel
SuperMultiporePW-N 
TSKgel
SuperMultiporePW-M 
TSKgel
SuperMultiporePW-H 

Base material: polymethacrylate polymethacrylate polymethacrylate

Particle size: 4 µm 5 µm 8 µm (6-10 µm)

Exclusion limit (Da):   120,000 1,000,000 10,000,000

 
Separation range (Da)
(PEO,PEG):
300 ~ 50,000 500,000  ~ 1,000,000 1,000~10,000,000
Theoretical plates:
> 16,000 > 12,000 > 7,000


 
 Literature:

TSKgel SEC Columns Brochure

Application Note - Analysis of water soluble polymers

 Application
Analysis of polyvinylpyrrolidone

Analysis of polyvinylpyrrolidone


MW calibration curve 

Calibration curve TSK-GEL SuperMultiporePW

P/N Description Particle size

Dimension

22789 TSKgel SuperMultiporePW-N 4 μm 6 mm ID x 15 cm L


22790 TSKgel SuperMultiporePW-M



5 μm 6 mm ID x 15 cm L

22791 TSKgel SuperMultiporePW-H


8 μm (6-10 μm) 6 mm ID x 15 cm L


22793 TSKgel SM(PW)-N Guard Column for P/N 22989


5 μm 4.6 mm ID x 3.5 cm L


22795 TSKgel SM(PW)-H Guard Column for P/N 21873, 21874, 21875

8 μm (6-10 μm)   4.6 mm ID x 3.5 cm L





EcoSEC SEC/GPC System

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Technical support

General Principles of
Chromatography - Mode Poster


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Tosoh Bioscience GmbH Zettachring 6, 70567 Stuttgart, Germany  Tel: +49-711-132570  Email: info.tbg@tosoh.com