TSK-GEL PWxl columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for PWxl columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSK-GEL PW page for typical samples and suitable eluents. Figure 1 shows the calibration curves for all PWxl-type columns. Figure 2 shows the benefits of PWxl columns over PW columns, namely the improved resolution due to the smaller particle sizes used in PWxl columns.
| Figure 1: Polyethylene glycol and oxide calibration curves on TSK-GEL PWxl columns |
Figure 2: Faster analysis and higher resolution with TSK-GEL PWxl columns |
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Although proteins can be analyzed on PWxl columns, pore volumes are much smaller than on SW-type columns. Figure 3 demonstrates the limited separation power of PWxl for protein samples.
Specialty Columns
TSKgel G-DNA-PW is available for the analysis of DNA fragments. Similarly, TSKgel G-Oligo-PW is the preferred column for the analysis of oligosaccharides. The mixed-mode column, TSKgel GMPWxl, has an extended linear calibration range, suitable for samples with a broad MW distribution and unknowns.
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Figure 3: Protein Calibration curves for TSK-GEL PWxl |
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