TSK-GEL PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.
| Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns |
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The figure to the right shows the calibration curves for all PW-type columns. Suitable solvents for various polymer samples are shown in the table below.
Specialty PW-type Columns
The mixed-mode column, TSKgel GMPW, has an extended linear calibration range, suitable for samples with a broad MW distribution, as well as unknown samples.
TSKgel G2000PW, the larger particle size equivalent of TSKgel G-Oligo-PW, is most suitable for semipreparative and preparative isolation of oligosaccharides.
 Recommended eluents for GFC of water-soluble polymers on TSK-GEL PW-type columns |
|
Type of polymer |
Typical sample
|
Suitable eluent
|
|
Nonionic hydrophilic |
polyyethylene glycol soluble starch, methyl cellulose, pullulan dextran, hydroxyethyl cellulose polyvinyl alcohol, polyacrylamide |
distilled water 0.01 N NaOH 20% DMSO Buffer or salt solution (e.g. 0.1-0.5 M NaNo3) |
|
Nonionic hydrophobic |
polyvinylpyrrolidone |
Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 M NaNO3) |
|
Anionic hydrophobic |
sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate |
Buffer or salt solution (e.g. 0.1 M NaNO3) |
|
Anionic hydrophobic |
sulfonated lignin sodium salt, sodium polystyrenesulfonate |
Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 M NaNO3) |
|
Cationic hydrophilic |
|
0.5 M acetic acid with 0.3 M Na2SO4 or 0.8 M NaNO3 |
|
Cationic hydrophobic |
poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt |
0.5 M acetic acid with 0.3M Na2SO4 |
|
Amphoteric hydrophilic |
peptides, proteins, poly- and oligosaccharides, DNA, RNA |
Buffer or salt solution (e.g. 0.1 M NaNO3) |
|
Amphoteric hydrophobic |
blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides |
Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 M NaNO3 or 35-45% CH3CN in 0.1% TFA) |
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