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Tosoh Bioscience GmbH: The Chemistry of Innovation
Size Exclusion: Gel Filtration Chromatography
TSK-GEL Columns
Proteins (biopolymers)
Organic-soluble Polymers
Water-soluble Polymers
TSKgel PW
TSKgel PWxl
TSKgel PWxl-CP
TSKgel SuperMultiporePW
TSKgel SuperOligoPW
Polar Organic-Soluble Polymers

Tosoh's polymer-based gel filtration columns are either prepared from polymethacrylate resin (TSKgel PW, PWXL and PWXL-CP) or from hydroxylated polyvinyl (TSKgel Alpha and SuperAW) resin. Each resin type can be synthesized in a wide variety of different pore sizes. The four major product groups also differ from one another in average particle size. A more important distinction is, however, the range of solvents that can be used with each product line.

Hydroxylated polymethacrylate beads

  • From water to 20% (under some conditions 50%) organic solvents

    • TSKgel PWXL and
      TSKgel PWXL-CP, 7-13 μm
    • TSKgel PW, 12-17 μm

Hydroxylated polyvinyl beads

  • Water and most polar organic solvents

    • TSKgel SuperAW, 4-9 μm
    • TSKgel Alpha, 7-13 μm

Each product group contains columns with distinctly different pore sizes, and one mixed-bed column

 

 


 

 

 Highlights

TSKgel PW, PWXL and PWXL-CP columns:

  • Hydrophilic methacrylate beads.
  • Stable in the pH range of 2 to 12
  • TSKgel PWXL-CP columns offer high recoveries for cationic polymers
  • TSKgel G6000PW is available in PEEK column hardware for ultra-low sample adsorption during virus analysis.
  • Temperatures up to 80˚C (50˚C for TSKgel G-DNA-PW)

TSKgel PWxl-CP columns

  • Minimal adsorption of cationic polymers

TSKgel SuperMultiporePW columns

  • Polymethacrylate beads
  • Semi-micro SEC
  • Multi-pore particle technology
  • Near linear calibration
  • Fast analysis
  • Smooth peak shapes

TSKgel SuperOligoPW columns

  • Semi-micro SEC
  • Fast analysis of low molecular weight oligomers
  • 3 µm monodisperse particles

TSKgel PW and PWXL

TSKgel PWxl-CP

Seven TSKgel PW columns vary in pore size from less than 100 Å to more than 1000 Å. A mixed-bed column is also available.

TSKgel PWXL columns cover the same pore size ranges as PW, but have smaller particle sizes and are thus more efficient. The TSKgel PWXL product line also offers specialty columns for analyzing:

 

TSKgel PWxl-CP columns have the same base resin as the PWxl columns and were specifically developed for the analysis of water-soluble cationic polymers and allow elution under low salt conditions.

Avaliable PWxl-CP-type columns and pore sizes:

  • TSKgel G3000PWxl-CP  [200Å]
  • TSKgel G5000PWxl-CP  [1000Å]
  • TSKgel G6000PWxl-CP  [>1000Å]


General Principles of
Chromatography - Mode Poster


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Tosoh Bioscience GmbH Zettachring 6, 70567 Stuttgart, Germany  Tel: +49-711-132570  Email: info.tbg@tosoh.com