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Tosoh Bioscience GmbH: The Chemistry of Innovation
Size Exclusion: Gel Filtration Chromatography
TSK-GEL Columns
Proteins (biopolymers)
Organic-soluble Polymers
Water-soluble Polymers
PW
PWxl
PWxl-CP for Cationic Polymers
Polar Organic-Soluble Polymers

Tosoh's polymer-based gel filtration columns are either prepared from polymethacrylate resin (PW, PWXL and PWXL-CP) or from hydroxylated polyvinyl (Alpha and SuperAW) resin. Each resin type can be synthesized in a wide variety of different pore sizes. The four major product groups also differ from one another in average particle size. A more important distinction is, however, the range of solvents that can be used with each product line.

Hydroxylated polymethacrylate beads

  • From water to 20% (under some conditions 50%) organic solvents
    • PWXL and PWXL-CP, 7-13 μm
    • PW, 12-17 μm

Hydroxylated polyvinyl beads

  • Water and most polar organic solvents
    • SuperAW, 4-9 μm
    • Alpha, 7-13 μm

Each product group contains columns with distinctly different pore sizes, and one mixed-bed column

 

 Highlights

PW, PWXL and PWXL-CP columns:

  • Hydrophilic methacrylate beads.
  • Stable in the pH range of 2 to 12
  • PWXL-CP columns offer high recoveries for cationic polymers
  • G6000PW is available in PEEK column hardware for ultra-low sample adsorption during virus analysis.
  • Temperatures up to 80˚C (50˚C for TSKgel G-DNA-PW)

PWxl-CP columns

  • Minimal adsorption of cationic polymers.

TSK-GEL PW and PWXL

TSK-GEL PWxl-CP

Seven TSK-GEL PW columns vary in pore size from less than 100 Å to more than 1000 Å. A mixed-bed column is also available.

TSK-GEL PWXL columns cover the same pore size ranges as PW, but have smaller particle sizes and are thus more efficient. The PWXL product line also offers specialty columns for analyzing:

 

TSK-GEL PWxl-CP columns have the same base resin as the PWxl columns and were specifically developed for the analysis of water-soluble cationic polymers and allow elution under low salt conditions.

Avaliable PWxl-CP-type columns and pore sizes:

  • G3000PWxl-CP  [200Å]
  • G5000PWxl-CP  [1000Å]
  • G6000PWxl-CP  [>1000Å]


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Tosoh Bioscience GmbH Zettachring 6, 70567 Stuttgart, Germany  Tel: +49-711-132570  Email: info.sep.eu@tosoh.com