TSKgel SuperSW columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology. 

Particle Characteristics
TSKgel SuperSW-type columns contain smaller particles than SWXL-type columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8 mm ID to 4.6 mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use.

HPLC System Requirements
It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on the TSKgel SuperSW columns. See Separation Report 95 for recommendations.

Calibration Curves The figure to the right shows the calibration curves for globular proteins for the two TSKgel SuperSW-type columns in comparison with those for the corresponding TSKgel SWXL columns. The following table summarizes the molecular weight ranges for TSKgel SuperSW2000 and TSKgel SuperSW3000 columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the pores. For comparative purposes, similar data is provided as well for TSKgel SW and SWXL-type columns.

Properties and separation ranges for TSKgel SW-type packings      (Molecular Weight in Da)
TSKgel packing	Particle size (µm)	Pore size (Å)	Globular proteins	Dextrans	Polyethylene  glycols and oxides
TSKgel SuperSW2000	4	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
TSKgel G2000SWxl/ TSKgel BioAssist G2SWxl	5	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
TSKgel QC-PAK GFC200	5	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
TSKgel G2000SW	10, 13	125	5,000 - 1 x 10^5	1,000 - 3 x 10^4	500 - 15,000
TSKgel SuperSW3000	4	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
TSKgel G3000SWxl/ TSKgel BioAssist G3SWxl	5	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
TSKgel QC-PAK GFC300	5	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
TSKgel G3000SW	10	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
TSKgel G4000SWxl/ TSKgel BioAssist G4SWxl	8	450	2 x 10^4 - 7 x 10^6	4,000 - 5 x 10^5	2,000 - 2.5 x 10^5
TSKgel G4000SW	13, 17	450	2 x 10^4 - 7 x 10^6	4,000 - 5 x 10^5	2,000 - 2.5 x 10^5
Data generated using the following conditions: Columns: Two 4 µm, 4.6 mm ID x 30 cm L TSKgel SuperSW columns in series; two 5 µm, 7.8 mm ID x 30 cm L TSKgel SWXL columns in series; two 10 µm, 7.5 mm ID x 60 cm L TSKgel SW columns in series Elution: Globular proteins: 0.3 M NaCl in 0.1 M (0.05 M for TSKgel SWXL columns) phosphate buffer, pH 7.0 Dextrans and polyethylene glycols and oxides (PEOs): distilled water