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Protein Calibration curves for TSKgel SWXL columns TSKgel SWXL columns, introduced in 1987, represent the 2nd generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
Particle Characteristics
TSKgel SWXL columns contain smaller particles than SW columns; 5 and 8 µm versus 10 and 13 µm. As the TSKgel SW-type columns, TSKgel SWXL columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SWXL columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharma products.
Calibration Curves
The figure to the right shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SWXL columns. The following table summarizes the molecular weight ranges for TSKgel G2000SWXL, TSKgel G3000SWXL and TSKgel G4000SWXL columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the pores. For comparative purposes, similar data is provided as well for TSKgel SW and TSKgel SuperSW columns.
 Properties and separation ranges for TSKgel SW-type packings
(Molecular Weight in Da) |
| TSKgel packing |
Particle
size (µm) |
Pore size
(Å) |
Globular proteins
|
Dextrans
|
Polyethylene glycols and oxides
|
| TSKgel SuperSW2000 |
4 |
125 |
5,000 - 1.5 x 10^5 |
1,000 - 3 x 10^4 |
500 - 15,000
|
TSKgel G2000SWxl/
TSKgel BioAssist G2SWxl |
5 |
125 |
5,000 - 1.5 x 10^5 |
1,000 - 3 x 10^4 |
500 - 15,000
|
| TSKgel QC-PAK GFC200 |
5 |
125 |
5,000 - 1.5 x 10^5 |
1,000 - 3 x 10^4 |
500 - 15,000
|
| TSKgel G2000SW |
10, 13 |
125 |
5,000 - 1 x 10^5 |
1,000 - 3 x 10^4 |
500 - 15,000
|
| |
TSKgel SuperSW3000
|
4 |
250 |
1 x 10^4 - 5 x 10^5 |
2,000 - 7 x 10^4 |
1,000 - 3.5 x 10^4
|
TSKgel G3000SWxl/
TSKgel BioAssist G3SWxl |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
2,000 - 7 x 10^4 |
1,000 - 3.5 x 10^4
|
| TSKgel QC-PAK GFC300 |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
2,000 - 7 x 10^4 |
1,000 - 3.5 x 10^4
|
| TSKgel G3000SW |
10 |
250 |
1 x 10^4 - 5 x 10^5 |
2,000 - 7 x 10^4 |
1,000 - 3.5 x 10^4
|
| |
TSKgel G4000SWxl/
TSKgel BioAssist G4SWxl |
8 |
450 |
2 x 10^4 - 7 x 10^6 |
4,000 - 5 x 10^5 |
2,000 - 2.5 x 10^5
|
| TSKgel G4000SW |
13, 17 |
450 |
2 x 10^4 - 7 x 10^6 |
4,000 - 5 x 10^5 |
2,000 - 2.5 x 10^5
|
Data generated using the following conditions:
| Columns: |
Two 4 µm, 4.6 mm ID x 30 cm L TSKgel SuperSW columns in series; two 5 µm, 7.8 mm ID x 30 cm L TSKgel SWXL columns in series; two 10 µm, 7.5 mm ID x 60 cm L TSKgel SW columns in series |
| Elution: |
Globular proteins: 0.3 M NaCl in 0.1 M (0.05 M for TSKgel SWXL columns) phosphate buffer, pH 7.0
Dextrans and polyethylene glycols and oxides (PEOs): distilled water |
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General Principles of
Chromatography - Mode Poster
Characterizing Biotherapeutics with HPLC - smart solutions for large molecules - Application note
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