TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

Particle Characteristics
TSKgel SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.

Calibration Curves
The figure below shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW-type columns. The following table summarizes the molecular weight ranges for TSKgel G2000SW, TSKgel G3000SW and TSKgel G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSKgel SWxl and TSKgel SuperSW-type columns.

Polyethylene oxide, dextran and protein calibration curves for TSKgel SW         columns

Properties and separation ranges for TSKgel SW-type packings      (Molecular Weight in Da)
TSKgel packing	Particle size (µm)	Pore size (Å)	Globular proteins	Dextrans	Polyethylene glycols and oxides
TSKgel SuperSW2000	4	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
TSKgel G2000SWxl/ TSKgel BioAssist G2SWxl	5	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
TSKgel QC-PAK GFC200	5	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
TSKgel SuperSW3000	4	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
TSKgel G3000SWxl/ TSKgel BioAssist G3SWxl	5	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
TSKgel QC-PAK GFC300	5	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
TSKgel G3000SW	10	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
TSKgel G4000SWxl/ TSKgel BioAssist G4SWxl	8	450	2 x 10^4 - 7 x 10^6	4,000 - 5 x 10^5	2,000 - 2.5 x 10^5
TSKgel G4000SW	13, 17	450	2 x 10^4 - 7 x 10^6	4,000 - 5 x 10^5	2,000 - 2.5 x 10^5
Data generated using the following conditions: Columns: Two 4 µm, 4.6 mm ID x 30 cm L TSKgel SuperSW columns in series; two 5 µm, 7.8 mm ID x 30 cm L TSKgel SWXL columns in series; two 10 µm, 7.5 mm ID x 60 cm L TSKgel SW columns in series Elution: Globular proteins: 0.3 M NaCl in 0.1 M (0.05 M for TSKgel SWXL columns) phosphate buffer, pH 7.0 Dextrans and polyethylene glycols and oxides (PEOs): distilled water