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Protein Calibration curves for TSK-GEL SWXL columns TSK-GEL SWXL columns, introduced in 1987, represent the 2nd generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
Particle Characteristics
TSK-GEL SWXL columns contain smaller particles than SW columns; 5 and 8 µm versus 10 and 13 µm. As the SW-type columns, TSK-GEL SWXL columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSK-GEL SWXL columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharma products.
Calibration Curves
The figure to the right shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSK-GEL SWXL columns. The following table summarizes the molecular weight ranges for G2000SWXL, G3000SWXL and G4000SWXL columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the pores. For comparative purposes, similar data is provided as well for TSK-GEL SW and SuperSW columns.
 Properties and separation ranges for TSK-GEL SW-type packings
(Molecular Weight in Da) |
| TSK-GEL packing |
Particle
size (µm) |
Pore size
(Å) |
Globular proteins
|
Dextrans
|
Polyethylene glycols and oxides
|
| SuperSW2000 |
4 |
125 |
5,000 - 1.5 x 10^5 |
1,000 - 3 x 10^4 |
500 - 15,000
|
G2000SWxl/
BioAssist G2SWxl |
5 |
125 |
5,000 - 1.5 x 10^5 |
1,000 - 3 x 10^4 |
500 - 15,000
|
| QC-PAK TSK200 |
5 |
125 |
5,000 - 1.5 x 10^5 |
1,000 - 3 x 10^4 |
500 - 15,000
|
| G2000SW |
10, 13 |
125 |
5,000 - 1 x 10^5 |
1,000 - 3 x 10^4 |
500 - 15,000
|
| |
SuperSW3000
|
4 |
250 |
1 x 10^4 - 5 x 10^5 |
2,000 - 7 x 10^4 |
1,000 - 3.5 x 10^4
|
G3000SWxl/
BioAssist G3SWxl |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
2,000 - 7 x 10^4 |
1,000 - 3.5 x 10^4
|
| QC-PAK TSK300 |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
2,000 - 7 x 10^4 |
1,000 - 3.5 x 10^4
|
| G3000SW |
10 |
250 |
1 x 10^4 - 5 x 10^5 |
2,000 - 7 x 10^4 |
1,000 - 3.5 x 10^4
|
| |
G4000SWxl/
BioAssist G4SWxl |
8 |
450 |
2 x 10^4 - 7 x 10^6 |
4,000 - 5 x 10^5 |
2,000 - 2.5 x 10^5
|
| G4000SW |
13, 17 |
450 |
2 x 10^4 - 7 x 10^6 |
4,000 - 5 x 10^5 |
2,000 - 2.5 x 10^5
|
Data generated using the following conditions:
| Columns: |
Two 4 µm, 4.6 mm ID x 30 cm L TSK-GEL SuperSW columns in series; two 5 µm, 7.8 mm ID x 30 cm L TSK-GEL SWXL columns in series; two 10 µm, 7.5 mm ID x 60 cm L TSK-GEL SW columns in series |
| Elution: |
Globular proteins: 0.3 M NaCl in 0.1 M (0.05 M for SWXL columns) phosphate buffer, pH 7.0
Dextrans and polyethylene glycols and oxides (PEOs): distilled water |
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General Principles of
Chromatography - Mode Poster
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